Colorimetric biosensor

Jelinek R, Kolusheva S. Polymerized hpid vesicles as colorimetric biosensors for biotechnological applications. Biotechnol Adv 2001 19 109-118. 

Lee NY, Jung YK, Park HG. On-chip colorimetric biosensor based on polydiacetylene (PDA) embedded in photopolymerized poly(ethylene glycol) diacrylate (PEG-DA) hydrogel. 

A software sensor for on-line determination of substrate was developed based on a model for fed-batch alcoholic fermentation process and on-line measured signals of ethanol, biomass, and feed flow. The ethanol and biomass signals were obtained using a colorimetric biosensor and an optical sensor developed in previous works that permitted determination of ethanol at a concentration of 0-40 g/L and biomass of 0-60 g/L. The volume in the fermentor could be continuously calculated using the total measured signal of the feed flow. The results obtained show that the model used is adequate for the proposed software sensor and determines continuously the substrate concentration with efficiency and security during the fermentation process. 

Liu, J., Lu, Y. (2004). Adenosine-dependent assembly of aptazyme-functionalized gold nanoparticles and its application as a colorimetric biosensor. Anal Chem 76, 1627-1632. 

Zhao, W. A., Chiuman, W., Brook, M. A., Li, Y. F. (2007). Simple and rapid colorimetric biosensors based on DNA aptamer and noncrosslinking gold nanoparticle aggregation. Chembiochem 8, 727-731. 

Lee E, Kim J, Myung J, Kang Y (2013) Modification of block copolymer photonic gels for colorimetric biosensors. Macromol Res 20 1219... 

Ou,L. J., JinJ. Y.,etal. Sensitiveand Visual Detection of Sequence-Specific DNA-Bind-ing Protein via a Gold Nanoparticle-Based Colorimetric Biosensor. "Analytical Chemis-hy,82(14), 6015-6024 (2010). 

The recognition of a ligand by a membrane-associated receptor or an enzyme (covalently or non-covalently incorporated into the PDA scaffold) provides die needed driving force to induce chromatic transition of PDA upon Ae occurrence of the interfacial binding event (i.e., biochromism), leading to die birdi of colorimetric biosensors for influenza virus, bacterial toxins and E. coli (4-6). 

One fundamental consideration in designing biosensors is to establish an effective signal transduction pathway upon the interaction of incorporated receptors with analytes at the detection interface. For PDA-based colorimetric biosensors, this requirement transforms into a bdlance between die rigidity (which determines the extent of polymerization as well as the signal transduction efficiency) and flexibility (which is necessary for effective binding... 

Su YL, Li JR, Jiang L, Cao J. Biosensor signal amphfication of vesicles functionalized with glycolipid for colorimetric detection of Escherichia coli. J Colloid Interface Sci 2005 284 114-119. 

Several techniques have been developed for the determination of purine and pyrimidine derivatives in food sample and in particular for hypoxanthine quantification biosensors (220-223) and electrochemical methods making use of immobilized enzyme electrode (224 -227), electrochemical enzymatic-based HA methods (228,229), enzyme reaction with fluorimetric detection (230), radioimmunoassay (231), colorimetric methods (232), capillary electrophoresis (233), and TLC (234). Many HPLC methods have also been developed and are reported in Table 4 (235-247) the most recent ones are described next. 

Protein phosphatase inhibition is usually detected by colorimetric methods, but the development of a biosensor requires the search of other transduction techniques. Electrochemistry has been widely used in biosensors because of the simplicity, easy to use, portability, disposability and cost-effectiveness of the devices. As protein phosphatase is not an oxidoreductase enzyme, our work has been devoted to the investigation of novel enzymatic substrates, electrochemically active only after their dephosphorylation by the protein phosphatase. Nevertheless, colorimetric assays have been used for the optimisation of several experimental parameters. 

Results obtained with the electrochemical biosensor were compared to those obtained from the colorimetric PPI assay with the enzyme in solution and by HPLC (see Table 21.1 of Procedure 21 in CD accompanying this book). All real samples contained microcystin at levels detectable by the amperometric biosensor and the colorimetric PPI... 

When the amperometric biosensor was used, results correlated with those obtained by colorimetric methods. The two sensitive enzymes allowed the detection of 0.5 nM anatoxin-a(s) and the limits of detection obtained with the two insensitive mutants were 16- and 50-fold higher than those obtained with the sensitive ones. 

MC-LR equivalent concentrations (pgg-1 dry weight) in cyanobacterial cells derived by the colorimetric PP2A inhibition assay, the amperometric PP2A inhibition-based biosensor and HPLC. In parentheses, the RSD values are... 

Cunningham B, Qiu J, Li P, Lin B (2002) Enhancing the surface sensitivity of colorimetric resonant optical biosensors. Sensors Actuators B Chem 6779 1-6... 

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