Colony polymerase chain reaction

Palmer, L. M., and Colwell, R. R. (1991). Detection of luciferase gene sequence in nonluminescent Vibrio cholerae by colony hybridization and polymerase chain reaction. Appl. Environ. Microbiol. 57 1286-1293. 

Gussow, D. and Clackson, T. (1989) Direct clone characterization from plaques and colonies by the polymerase chain reaction. Nucleic Acids Res 17, 4000. 

After both selective regimes, microbes were isolated as single colonies, DNA isolated and primers for 16S rRNA genes were used to amplify, fragments with polymerase chain reactions as previously described" Comparisons of the DNA sequence from the various colonies with those in DNA sequence data bases allowed the identification of the recovered microbes. 

CDC Centers for Disease Control and Prevention CFU colony forming unit CNS central nervous system CSF cerebrospinal fluid CT computed tomographic DIC disseminated intravascular coagulation EV-PCR enterovirus-specific reverse-transcriptase polymerase chain reaction... 

LaPointe, G., Leblanc, D. Morin, A., Use of a polymerase-chain-reaction-amplified DNA probe from Pseudomonas putida to detect D-hydantoinase-producing microorganisms by direct colony hybridization. Appl. Microbiol. Biotechnol., 42 (1995) 895-900. 

---