Collagen antibodies

Chintalacharuvu SR, Wang JX, Giaconia JM, Venkataraman C. An essential role for CCL3 in the development of collagen antibody-induced arthritis. Immunol Lett 2005 100(2) 202-204. 

Concentrations of anti-collagen antibodies can vary between mice, so it is important to test a range of concentrations of serum from each mouse in one ELISA. A starting dilution of 1 50 is recommended, with seven 4-fold dilution steps. 

In the collagen antibody-ELISA, normal mouse serum can be included as an additional negative control in the same dilution range as sera from mice with CIA. This serum should not react in this ELISA. 

Tanaka D, Kagari T, Doi H, Shimozato T. Administration of anti-Type II collagen antibody sustains footpad swelling of mice caused by a delayed-type hypersensitivity reaction and induces severe arthritis. Clin Exp Immunol 2007 48 360-367. 

Ibrutinib was also active in animal models of several autoinunune disorders such as rheumatoid arthritis and lupus. The racemic version of ibratinib can completely suppress disease progression in a collagen antibody-induced arihriiis model. Ibrutinib itself is also active in arthritis and lupus mouse models by suppressing autoantibody production." ... 

Fig. 8. Immunofluorescent examination of human skin (single sample, 22 months) for distribution of different collagen types, showing (A) reticular dermis examined with anti-lype I collagen antibody, (B) reticular dermis examined with anti-type III collagen antibody, (C) papillary dermis examined with anti—type III antibody, and (D) dermal/epidermal jimction examined with anti-type IV collagen antibody.

Figure 8. Scatter plot demonstrating the inverse correlation (dashed line of best fit r value = 0.77 p < 0.05) between IgG anti-Collagen antibodies and GTase activity in the serum of collagen immunized arthritic DBA/1 mice.

In addition to antibodies targeting the CD3 subunit of the TCR complex, antibodies against the a and (3 subunits of the TCR have been tested as therapeutic agents. A benefit for the treatment experimental autoimmune encephalomyelitis or collagen induced arthritis could be shown in animal models [8]. 

Antibodies antibody fragments (e.g., collagen-specific drug-toxin conjugates)... 

Pristane (2,6,10,14-tetramethylpentadecane) is a mineral oil known to induce arthritis, a disease also referred to as pristane-induced arthritis (PIA) [58], Susceptibility to PIA is MHC-haplotype dependent, in that DBA/1 (H2q) mice are susceptible whereas DBA/2 (H2d) are not, and is accompanied by a broad spectrum of autoantibodies, including anti-Rheuma Factor (RF), anti-collagen and antibodies to heat shock proteins (HSP). PIA is clearly immune dependent since nu/nu mice and irradiated mice do not develop PIA. PIA involves polyclonal T cell activation [59], particularly CD4+ cells [58], Intriguingly, mice can be protected from developing PIA by HSP65-specilic CD4+ Th2 cells [60],... 

In living systems, proteins function as catalysts (enzymes), for defense (antibodies, immunoglobulins), signal transduction (hormones, receptors), metabolic regulation (hormones, enzymes, receptors, ion channels), movement (microtubules), and architecture (structural proteins such as collagen). 

Furthmayr, H. (1982). Immunization procedures, isolation by affinity chromatography and seriological and immunochemical characterization of collagen-specific antibodies. In Furthmayr, H., ed. immunochemistry of the Extracellular Matrix, Vol. 1. CRC Press, Boca Raton, FL. 

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