Genomic libraries cloning

In a variation of genomic libraries, clone libraries of complementary DNA (cDNA) molecules, called cDNA libraries, are produced from mRNA molecules by reverse transcription. This technique can be used to evaluate the transcriptome of certain cell types under... 

Many clones must be created to be confident that the genomic library contains the gene of interest. The probability, P, that some number of clones, N, contains a particular fragment representing a fraction, f, of the genome is... 

Library A collection of cloned fragments that represents the entire genome. Libraries may be either genomic DNA (in which both introns and exons are represented) or cDNA (in which only exons are represented). 

At this time, more then thirty channel DNAs have been cloned and characterized from various sources, predominantly from Drosophila melanogaster, mouse, rat and human cDNA/genomic libraries [6-31]. Inspection of the derived primary K channel protein sequences indicates that voltage-gated channels belong to a... 

Genomic library A collection of clones made from a set of randomly generated overlapping DNA fragments representing the entire genome of an organism. 

Genomic libraries are also useful to clone and study DNA sequences that are not expressed in cells (response elements, introns,promoters). 

A DNA library is a collection of cloned restriction fragments of the DNA of an organism. Two kinds of libraries will be discussed genomic libraries and cDNA libraries. Genomic libraries ideally con-... 

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