Cloned and sequenced

Several silk fibroin genes have been cloned and sequenced and they all show a similar sequence pattern variable domains at the N- and C-termini flank a large region of repetitive short sequences of alternating poly-Ala (8 to 10 residues) and Gly-Gly-X repeats (where X is usually Ser, Tyr, or Gin). This middle region varies in length and may comprise up to 800 residues. 

Type II restriction enzymes have received widespread application in the cloning and sequencing of DNA molecules. Their hydrolytic activity is not ATP-depen-dent, and they do not modify DNA by methylation or other means. Most importantly, they cut DNA within or near particular nucleotide sequences that they specifically recognize. These recognition sequences are typically four or six nucleotides in length and have a twofold axis of symmetry. For example, E. coU has a restriction enzyme, coRI, that recognizes the hexanucleotide sequence GAATTC ... 

According to Markova et al. (2004), the cDNA encoding the luciferase of Metridia longa was cloned and sequenced. The luciferase is a 219-amino acid protein with a molecular weight of 23,885. 

Inouye, S., and Tsuji, F. I. (1993). Cloning and sequence analysis of cDNA for the Ca2+-activated photoprotein, clytin. FEBS Lett. 315 343-346. 

Ye, L., Buck, L. M., Schaeffer, H. J., and Leach, F. R. (1997). Cloning and sequencing of a cDNA for firefly luciferase from Photuris pennsylvanica. Biochim. Biophys. Acta 1339 39-52. 

Rebmann G. Hertig C. Bull J. Mauch F. Dudler R. (1991) Complementary DNA cloning and sequence analysis of a pathogen-induced putative peroxidase from rice / / Plant Mol. Biol. V. 16. P. 329-331. 

Roberts E. Kutchan T. Kolattukudy P. E. (1988) Cloning and sequencing of cDNA for a highly anionic peroxidase from potato and the induction of its mRNA in suberizing potato tubers and tomato fruits / / Plant Mol. Biol. V. 11. P. 15-26... 

Venom Allergen Function/ name MW kDa Major/ minor Cloned and sequenced Expressed as recombinant Glycosylated (n sites)... 

Knowledge of DNA sequences permits deduction of the primary structures of polypeptides. DNA sequencing requires only minute amounts of DNA and can readily yield the sequence of hundreds of nucleotides. To clone and sequence the DNA that encodes a partic-... 

The genes encoding the polypeptide backbones of a number of mucins derived from various tissues (eg, pancreas, small intestine, trachea and bronchi, stomach, and salivary glands) have been cloned and sequenced. These studies have revealed new information about the polypeptide backbones of mucins (size of tandem repeats, potential sites of N-glycosylation, etc) and ultimately should reveal aspects of their genetic control. Some important properties of mucins are summarized in Table 47-8. 

Takahashi H, B Kimura, M Yoshikawa, T Fuji (2003) Cloning and sequencing of the histidine decarboxylase genes of Gram-negative, histamine-producing bacteria and their application in detection and identification of these organisms in fish. Appl Environ Microbiol 69 2568-2579. 

Wong CM, MJ Dilworth, AR Glenn (1994) Cloning and sequencing show that 4-hydroxybenzoate hydroxylase Poba is required for uptake of 4-hydroxybenzoate in Rhizobium leguminosarum. Microbiology (UK) 140 2775-2786. 

Myers CR, ID Myers (1997) Cloning and sequence of cymA, a gene encoding a tetraheme cytochrome c required for reduction of iron (III), fumarate, and nitrate by Shewanella putrefaciens MR-1. J Bacteriol 179 1143-1152. 

Blehert DS, BG Fox, GH Chambliss (1999) Cloning and sequence analysis of two Pseudomonas flavoprotein xenobiotic reductases./Bacreno/ 181 6254-6263. 

Ratnatilleke A, JW Vrijbloed, JA Robinson (1999) Cloning and sequencing of the coenzyme B,2-binding domain of isobutyryl-CoA mutase from Streptomyces cinnamonensis. Reconstitution of mutase activity and characterization of the recombinant enzyme produced in Escherichia coli. J Biol Chem 274 31679-31685. 

Okeke BC, YC Chang, M Hatsu, T Suzuki, K Takamizawa (2001) Purification, cloning, and sequencing of an enzyme mediating the reductive dechlorination of tetrachloroethene (PCE) from Clostridium bifermen-tans DPH-1. Can J Microbiol AT. 448-456. 

The enzymes involved in the degradation of phenanthrene by Nocardioides sp. strain KP7 have been characterized. In this strain, the genes are chromosomal, and phdA and phdB encoding the a- and P-subunits of the dioxygenase, phdC and phdD that encode the ferredoxin, and ferredoxin reductase have been cloned and sequenced (Saito et al. 2000). The order of the genes... 

Thibodeau J, A Gauthier, M Duguay, R Villemur, F Lepine, P Juteau, R Beaudet (2004) Purification, cloning, and sequencing of a 3,5-dichlorophenol reductive dehalogenase from Desulfitobacterium frappieri BCP-l. Appl Environ Microbiol 70 4532-4537. 

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