Citrate-phosphate-dextrose

We used outdated human CPD (citrate-phosphate-dextrose) blood from the Dayton Community Blood Center. At 21 days, CPD blood still retains 78% survival of the red blood cells and would fairly well simulate in vivo physiological conditions. During these tests, many enzymes and proteins may denature and/or precipitate. Even after suffering that trauma, the resulting fluid is more suitable for material testing than other pseudo-physiological fluids, since it still contains most of the salts, lipids, hormones, oligomers, nucleotides, saccharides, etc., found in whole blood in vivo. 

Whole blood is blood that has been aseptically withdrawn from humans. A suitable anticoagulant is added (often heparin or a citrate-dextrose-based substance), although no preservative is present. The blood is usually stored at temperatures ranging from 1-8 °C, and has a short shelf life (48 h after collection if heparin is used as the anticoagulant, or up to 35 days if citrate-phosphate-dextrose with adenine is employed). 

TAC of plasma of blood anticoagulated with CPDA-1 fluid (containing citrate, phosphate, dextrose, and adenine) and stored under blood-banking conditions at 3°C showed decreased TAC after the day 12 of storage. A decrease of TAC by approximately 25% was observed on day 16, and a decrease by over 30% was noted on days 20 and 25 (J4). 

Whole blood containing citrate phosphate dextrose anticoagulant and stored at 5°C was typed up to 10 months. 

Fitzgerald, R.D. Martin, C.M. Dietz, G.E. Doig, G.S. Potter, R.F. Sibbald, W.J. Transfusing red blood cells stored in citrate phosphate dextrose adenine-1 for 28 days fails to improve tissue oxygenation in rats. Crit. Care Med. 1997, 25, 726-732. 

Anticoagulant citrate phosphate dextrose adenine solution, USP... 

Citrate-phosphate-dextrose (CPD) medium is better than ACD for maintaining organic phosphate levels and for preventing a reduction in P50, probably because of the higher pH of CPD. In 1971, 90% of blood banks in the United States used ACD and only 10% used CPD by 1975, the reverse was true. The shelf life of cells is the same for both media... 

ThERap CAT Component of anticoagulant citrate soins (citrate dextrose soln citrate phosphate dextrose soln citric acid syrup),... 

Citrate phosphate Double dextrose/additive solution 3 Anticoagulant citrate phosphate 2X dextrose solution (CP2D) Use only with automated apheresis devices for collecting human blood and blood components 000127 Haemonetics Corp. 400 Wood Road Braintree, MA 02184 January 18, 2002... 

AS3 nutricel additive system additive solution formula 3 Anticoagulant citrate phosphate 2X dextrose solution To provide the CP2D during collection and add AS3 to the separated red blood cells in order to extend the length of time they can be stored 001214 Gambro BCT, Inc. 10811 W. Collins Ave. Lakewood, CO 80215 May 29, 2002... 

Abbreviations BA benzyl alcohol BHT butylated hydroxytoluene BKC benzalkonium chloride BzCI benethonium chloride CCS croscarmellose sodium cit citric acid dex dextrose IPM isopropyl myristate KP potassium phosphates MCC microcrystalline cellulose MeC methyl cellulose MePB methyl parabens Mr relative molecular mass NaCit Na citrate NaCMC Na carboxymethylcellulose Na2edta disodium edetate NaP sodium phosphate PEG polyethylene glycol PE-OH phenylethyl alcohol PG propylene glycol pov povidone PrPB propyl parabens SAI steroidal anti-inflammatory Tw Tween (polysorbate)... 

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