Chromosome aberration test INDEX

A detailed discussion of the test procedures employed is contained within Galloway et al. (1994). Detectable levels of chromosome aberrations are often found only at doses that induce some evidence of cytotoxicity, and consequently most current recommended protocols require that the maximum test concentration should induce >50% reduction in cell number or culture confluency for cell lines, or an inhibition of mitotic index by >50% for lymphocytes. However, there are growing concerns as to the relevance of genotoxic effects that are found only at highly cytotoxic concentrations (Galloway 2000), and this may be reflected by the poor specificity of mammalian cells tests (Kirkland et al. 2005). 

Different types of tests for genotoxicity of peroxisomicine Ai (T514), such as inhibition of mitotic index, lymphocyte proliferation kinetics, frequency of sister chromatid interchange, and frequency of chromosomic aberrations, have yielded negative results [75]. 

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