Chromosomal engineering

Matsubayashi, M. (1991). Phylogenetic relationships in the potato and its related species. In T. Tsuchiya, P. K. Gupta (Eds.), Chromosome Engineering in Plants Genetics, Breeding and Evolution, Part B (pp. 93-118). Elsevier, Amsterdam. 

Yu D, Ellis HM, Lee EC, Jenkins NA, Copeland NG, Court DL (2000) An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sd USA 97 5978-83. 

Wu YQ, Taliaferro CM. 2009. Bermudagrass. In Singh RJ (editor). Genetic Resources, Chromosome Engineering, and Crop improvement Forage Crops. CRC Press, pp. 229-273. 

Genetic Control. Manipulation of the mechanisms of inheritance of the insect pest populations has occurred most successhiUy through the mass release of steri1i2ed males, but a variety of other techniques have been studied, including the environmental use of chemostetilants and the mass introduction of deleterious mutations, eg, conditional lethals and chromosomal translocations (58 ndash 60) (see Genetic engineering). 

Leaf discs are floated on a suspension of Agrobacterium ceWs that contain genetically engineered Ti-plasmids. The bacterium infects cells around the edges of the discs, and the T-DNA is transferred to the chromosomes of the infected cells. 

Lycopene is a carotenoid with anticancer properties. To improve the production of lycopene by increasing the IPP flux in an engineered E. coli, the dxs gene was overexpressed and enhanced lycopene production was obtained [45]. In another example, the native promoters of DXP pathway genes in the E. coli chromosome were replaced with the strong bacteriophage T5 promoter (PTs), and the increase in isoprenoid precursors resulted in improved /3-carotene production (with a titer of 6 mg/g dry cell weight) [44]. 

Wang, Y. and Pfeifer, B.A. (2007) 6-Deoxyerythronolide B production through chromosomal localization ofthe deoxyerythronolide B synthase genes in E. coli. Metabolic Engineering, 10, 33-38. 

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