Chromatography, thin-layer ultra-violet

The conventional approach to solvent extraction is the batch method. Early work with this method was hampered by the low concentration of the compounds present and the relative insensitivity of the methods of characterization. Thus lipids and hydrocarbons have been separated from seawater by extraction with petroleum ether and ethyl acetate. The fractionation techniques include column and thin-layer chromatography with final characterisation by thin-layer chromatography, infrared, and ultra-violet spectroscopy and gas chromatography. Of these techniques, only gas chromatography is really useful at levels of organic matter present in seawater. With techniques available today such as glass capillary gas chromatography and mass spectrometry, much more information could be extracted from such samples [20]. 

Thin layer chromatography Thiocarbonylsulfanylpropionic acid Ultra violet spectroscopy 2-Methylpropionamidine dihydrochloride 2,2 -Azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride... 

Shellard, E. j., and M. Z. Alam The quantitative determination of some mitra-gyna oxindole alkaloids after separation by thin layer chromatography. Part IV. Comparison of ultra-violet spectrometry, colorimetry and densitometry as methods for the quantitative determination of oxindole alkaloids in plant material. J. Chromatog. 36, 72 (1968). 

D. M. Alperin, H. Carminatti, V. Idoyaga-Vargas, and R. O. Couso, Thermal-ultra-violet method for differential detection and recovery of organic compounds in thin-layer chromatography, J. Chromatogr. 265 193-200(1983). 

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