Chromatography on Ion-Exchange Resins

The proteins known today as linker or HI histones were initially described as the abundant lysine-rich nuclear proteins that could be separated by chromatography on ion exchange resin from other major basic nuclear proteins known today as core histones (for review see Refs. [1,2]). During gel electrophoresis of histones the HI fraction migrated as the slowest and most heterogeneous band. Upon the discovery of nucleosomal organization of chromatin in the mid 1970s it turned out that linker histones are not involved in the assembly of the nucleosomal protein core, but bind to DNA between nucleosomes (hence their name). 

R3. Richmond, V., and Caputto, R., Studies on the large molecular components of gastric juice chromatography on ion-exchange resins. Am. ]. Physiol. 179, 664-665 (1954). Abstr. 

Poly-0-(carboxymethyl) [9044-05-7] Displacing agent for proteins in displacement chromatography on ion exchange resins. 

Other possibilities eire automated column chromatography on ion-exchange resins/ high performance liquid chromatography (HPLC), or high resolution two-dimensional thin-layer chromatography (TLC). Purine bases and nucleosides are simultaneously detected. 

For preparative purposes, successful separation has been carried out by column chromatography on ion exchange resins (Amberlite IRC 50 and XE 64) and by electrophoresis in cellulose columns . 

Analysis of glycosaminoglycan hydrolyzates is often complicated by the presence of basic and acidic glycoses, in addition to neutral monosaccharides. Adsorption chromatography on silica gel was used to classify such a hydrolyzate, and the neutral fraction was shown to contain D-glucose, D-galactose, and D-mannose.288 Separation on ion-exchange resins may also be used.51,149 The resolution of the acidic and basic fractions is discussed in Sections IX (p. 71) and X (p. 78). 

Owing to their intrinsic basicity, marine invertebrate guanidine alkaloids have a rather polar behavior and their isolation from complex mixtures may be difficult. Isolation procedures frequently include chromatography on lipophilic Sephadex LH20, on reversed phase silica gel (such as Cig bonded, aminopropyl bonded, or cyanopropyl bonded), or even on ion-exchange resins. HPLC purification using acidic (TFA) or buffered eluents have frequently been employed. 

Kennedy, J.F., 1974. Methodology and instrumentation in the chromatography of carbohydrates on ion-exchange resins. Biochem. Soc. Trans., 2 54—64. 

B. Arwidi and O. Samuelson, Partition chromatography of sugars on ion-exchange resins, Sven. Kem. Tidskr., 1965, 77, 84-90 L.-I. Larsson and O. Samuelson, An automated procedure for separation of monosaccharides on ion exchange resins, Acta Chem. Scand., 1965, 19, 1357-1364. 

Fig. 3 Apparatus set for the determination of the adsorption model of adsorbate on ion exchange resin by inverse gas chromatography. (1), (7), (12), (21) Electromagnetic valve (2), (8), (13), (22) flow stabilizer, (3), (9), (14), (23) needle valve (4), (11), (24) six-port valve (5), (15), (25) injector (6), (16) sorption pipe (10) reservoir for vaporizing (17) thamal conductivity detector (18) amplifier (19) analog-to-digital converter (20) computer (26) chromatography column (27) flame ionization detector (28) freezing capillary (29) electrometer.

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