Chromatography on Exchange Resins

In fiuther work of these authors, chromatography on Amberlite IRC-50 XE-64 columns yielded two sharply delineated fractions differing in proteolytic activity curves (R4, R5, T3-T5a). Pepsin activity was eluted at effluent pH of 4.0, gastricsin at effluent pH of 4.4. Proteases eluted 

Faulkner (personal communication) in the same laboratory used column chromatography for clinical purposes and for fractionation of individual gastric juices. Nondialyzable solids from 50 ml gastric juice were applied to a 1 X 10 cm column and eluted with citrate and phosphate buflFers of increasing pH (3.2-8.0). Welsh et al. (W12, W12a) 

Subsequently, Welsh et al. (Wll) extended this work and found B12 binding in 3 of the protein peaks 1, 4, and 5 (Fig. 32). Paper electro- 

Wada et al. (W2) extended this work and studied by this method 

Vita et al. (V2), using highly basic Dowex-1 X2 resin in Cl and OH form, separated acidic aminopolysaccharide (hexuronic acid content of 35-37%) and neutral mucopolysaccharide hexosamine content 25-30%) from an aqueous extract of hog gastric mucosa. 

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