Gastric juice chromatography

R3. Richmond, V., and Caputto, R., Studies on the large molecular components of gastric juice chromatography on ion-exchange resins. Am. ]. Physiol. 179, 664-665 (1954). Abstr. 

Retief, F. P., Gottlieb, C. W., Kochwa, S., Pratt, P. W., and Herbert, V., Separation of vitamin Birbinding proteins of serum, gastric juice and saliva by rapid DEAE cellulose chromatography. Blood 29, 501-516 (1967). 

Fractionation of Macromolecular Components of Human Gastric Juice by Electrophoresis, Chromatography, and Other Physicochemical Methods... 

Bi2-Sepharose has also been used in the preparation of human intrinsic factor from gastric juice. Affinity chromatography was the sole purification technique and yielded a protein 853-fold purified [91]. A series of derivatives of B12, lacking the nucleotide portion of the molecule, coupled to Sepharose were used in the resolution of two hog B12 binding proteins, termed intrinsic and non-intrinsic factor. The total B12 binding protein population, from which the two forms were resolved, was isolated by affinity chromatography on B -Sepharose [92]. 

Richmond, Tang, Caputto, and Trucco, in Wolfs laboratory, using column chromatography, reported the successful separation of the two proteolytic enzymes from human gastric juice (R5, R6, T2-T6). Of these, one had optimum proteolytic activity at pH 2 (pepsin), while the other... 

The presence of peptides and amino acids in gastric juice was demonstrated many years ago by Nencki and Sieber (N3), Babkin (Bl), and Komarov (K22). This became better documented only more recently with the use of modem physicochemical tools, such as paper partition chromatography, electrophoresis, and polarography. Demonstration of the products of proteolytic degradation of serum proteins and muco-substances has also been made possible only by the use of these methods. The reader is referred to the second article by the author (G21) for pertinent information and to the article by Bouda (B29). 

This material, described by Nakahara and Fukuoka (Nl), is present only in cancer gastric juice. Upon intraperitoneal injection into mice, it depresses the catalase titer of the liver, kidney, and blood. The relationship of this material to mucopolysaccharides and mucoproteins of gastric juice is not well known. Toxohormone— like material obtained from normal stomach which is biologically inactive, however—was found to have lower polarographic activity than that from stomachs with gastric cancer (M18). Separation of materials with toxohormone activity by continuous electrophoresis on paper curtain and column chromatography is discussed in the following review in this volume. 

G21. Class, G. B. J., Fractionation of macromolecular components of the human gastric juice by electrophoresis, chromatography and other physico-chemical methods. Adv. Clin. Chem. 7, 373-479 (1964). 

FRACTIONATION OF MACROMOLECULAR COMPONENTS OF HUMAN GASTRIC JUICE BY ELECTROPHORESIS, CHROMATOGRAPHY. AND OTHER PHYSICOCHEMICAL METHODS ... 

Carbohydrate Spectrum of Some Fractions of Human Gastric Juice Resolved by Paper Electrophoresis on Column Chromatography... 

Faulkner (personal communication) in the same laboratory used column chromatography for clinical purposes and for fractionation of individual gastric juices. Nondialyzable solids from 50 ml gastric juice were applied to a 1 X 10 cm column and eluted with citrate and phosphate buflFers of increasing pH (3.2-8.0). Welsh et al. (W12, W12a)... 

Fig. 31. Chromatography of normal acid gastric juice oa Amberhte IRC-50 column. The chromatogram of a posthistamine stimulated acid-containing gastric juice specimen from a normal individual. There are 6 protein peaks peak II contains pepsin and peak III, gastricsin. Hexoses are always eluted with peak I, and almost always with peaks IV and V. Blood group substances A and B, when present, are eluted in peak I. From Welsh et al. (Wll).

Fig. 32. Chromatography of normal acid gastric juice on Amberlite IRC-50 column. Example of Co8 -vitamin Bjj binding of effluent obtained from column chromatographic separation of an individual gastric juice specimen. The vitamin Bjg binding is expressed as mug Co 0-vitamin Bjg bound/6 ml effluent. From Welsh et al. (Wll).

Gl. Gilligan, D. R., Moor, R. J., and Warren, S., Paper partition chromatography of free amino acids and peptides of normal human gastric juice. ], Natl. Cancer Inst. 12, 657-676 (1951). 

W2. Wada, T., Ibayashi, J., Soto, K., and Anzai, T., Studies on gastric juice protein. IV. Analytical characterization by means of cation exchange resin chromatography. Gann 52, 15-25 (1961). 

Heybroek, W.M. Caulfield, M. Johnston, A. Himer, P. Automatic on-line extraction coupled with electrochemical detection as an improved method for the HPLC co-analysis of codeine and morphine in plasma and gastric juice. J.Pharm.Biomed.Anal., 1990, 8, 1021-1027 Chen, Z.R. Bochner, F. Somog3d, A. Simultaneous determination of codeine, norcodeine and morphine in biological fluids by high-performance hquid chromatography with fluorescence detection. J.Chromatogr., 1989, 491, 367-378... 

Toreson, H. Eriksson, B.M. Determination of erythromycin in gastric juice and blood plasma by liquid chromatography and electrochemical detection. J.Chromatogr.B, 1995, 673, 81-89... 

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