Chromatography of enzymes

Hamada, J. S., Large-scale high-performance liquid chromatography of enzymes for food applications, /. Chromatogr. A, 760, 81, 1997. 

Lowe, C.R., and Dean, P.D.G. (1971) Affinity chromatography of enzymes on insolubilized cofactors. FEBS Lett. 14, 313-316. 

Figure 9.45 Chromatography of enzyme assay media. Peaks 1, aspartate 2, glutamate 3, asparagine 4, glutamine 5, Tris-HCl buffer. Elution profile of the assay medium incubated for (A) zero time and (fl) 30 minutes. (From Unnithan et al., 1984.)...

Figure 2. Oligosaccharide mapping of MAb MY9-6. CarboPac PAIOO chromatography of enzyme digests of MAb MY9-6. In Panels A, B, and C the substrate was 100 [ig of MAb My9-6. In Panels D, E, and F the substrate was a PNGase F digest derived from 100 /Xg of MAb MY9-6.

The quantitative separation of phosphorus acids and esters by ion-exchange chromatography has been reviewed. The separation of nucleoside polyphosphates by anion-exchange chromatography and the affinity chromatography of enzymes on immobilized adenosine monophosphate have also been described. 

R.R. Maestas, J.R. Prieto, G.D. Kuehn and J.H. Hageman. Polyacryamide-boronate beads saturated with biomolecules A new general support for affinity chromatography of enzymes. 

V. Bouriotis, I.J. Galpin and P.D.G. Dean. Applications of immobilized phenylboronic acids as supports for group-specific ligands in the affinity chromatography of enzymes. J. Chrom. 210 267-278 (1981). 

Ehle, H. Horn, A. Immunoaffinity chromatography of enzymes. Bioseparation 1990,1, 97-110. 

Affinity chromatography of enzymes from soybean and Takadiastase on glycose-substituted agaroses showed that the p-D-2-acetamido-2-deoxygluco-sidases are not adsorbed specifically, although some purification of these enzymes could be achieved. ... 

Friedberg F, Rhoads AR. Affinity chromatography of enzymes. In Hage DS, editor. Handbook of affinity chromatography 2nd ed. Boca Raton, FL CRC Press 2005. p. 313-46. 

A benzylated, cross-linked agarose gel has been prepared. Gels variously cross-linked with 2,3-dibromopropan-l-ol (Scheme 7) have been used in the chromatography of enzymes, including polysaccharide hydrolases. In some instances, the enzyme was denatured on elution from the gel, although the... 

---