Chromatography of DNA-Modifying Enzymes

6 Biochemical Applications of Process-Scale Ion-Exchange Liquid Chromatography 

This protocol gives a rapid and efficient single step purification of a group of commercially important bacterial enzymes. If further fractionation of these enzymes should be required then chromatography by anion-exchange may facilitate further purification, although in terms of the functional role of these enzymes may in fact be an unnecessary luxury. 

The author wishes to acknowledge the contributions of his colleagues within Whatman International Ltd. who carried out the work described in this review Stephen E. Badger, Brian N. Brook, Jayne A. Cox, Navin D. Pathirana, Michael Streater and David W. Toome based in Maidstone, UK, and Edward T. Butts, Mark L. Koscielny and Linda Lane of Whatman Inc. based in Fairfield, NJ, USA. Acknowledgement is made to John M. Ward and co-workers in the Department of Biochemistry, University College London, UK for collaboration in the studies of DNA-modifying enzymes. 

Preparative chromatography can be practised on two different scales laboratory (recovery of grams of sample) and production (recovery of kilograms of sample). Preparative Scale Supercritical Fluid Chromatography (PS-SFC) has been practised on the laboratory scale from the very beginning of SFC in 1962 [1], More information on these historical experiments has been gathered by Berger et al. [2] and Bevan [3]. 

PS-SFC can be described as a modification of preparative HPLC. Their principles differ on four major points  

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