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Chromatography HPLC, reversed phase

Steinbrech, B., Neugebauer, D., Zulauf, G. (1986). Determination of surfactants by liquid chromatography (HPLC). Reversed phase ion-pair chromatography of alkyl sulfates and alkyl sulfosuccinates. Analytische Chemie 324(2), 154—157. [Pg.445]

The resulting active fraction was further processed in five steps of high performance liquid chromatography (HPLC) reverse phase HPLC on Develosil 5Co (Nomura Kagaku) with 20-25% acetonitrile in 0.08% trifluoroacetic acid (TEA) (step 12), reverse phase HPLC on Hi-Pore RP-304 (Bio-Rad) with 20-40% acetonitrile in 0.08% TEA (step 13), reverse phase HPLC on Hi-Pore RP-304 with 20-40% acetonitrile in 0.1% heptafluorobutyric acid (HEBA), cation exchange HPLC on TSKgel (Toyo Soda) with... [Pg.21]

Due to high water solubility of monosaccharides, the use of the most routine high-performance liquid chromatography (HPLC) reversed-phase columns is also not suitable for their analysis. Extremely pure solvents have to be used if ultraviolet (UV) detection is applied. If a refractometer is used as the detector (RD), extremely steady chromatographic conditions are necessary. Nevertheless, HPLC is applied in the practice. The modern approach involves the use of propylamino columns (e.g.. Refs. 3 and 4). [Pg.310]

Reverse-Phase (RP) and High-Performance Liquid Chromatography (HPLC). Reverse-phase chromatography separates proteins based on the hydrophobicity of the molecules. It results in the separation of molecules based on the mass of proteins, because hydrophobicity of the... [Pg.69]

As a method of research, has been used high-performance liquid chromatography in reversed - phase regime (RP HPLC). The advantage of the present method is the following the additional information about AIST and FAS composition (homologous distribution) simple preparation of samples (dilution of a CS sample of in a mobile phase). [Pg.133]

Other groups have also used EC and CE to perform non-comprehensive multidimensional separations (15, 16). A three-dimensional separation was performed by Stromqvist in 1994, where size exclusion chromatography (SEC), reverse-phase HPLC, and CZE were used in an off-line manner to separate peptides (17). The most useful information gained from all of these non-comprehensive studies was knowledge of the orthogonality and compatibility of EC and CE. [Pg.203]

The TLC process is an off-line process. A number of samples are chromatographed simultaneously, side-by-side. HPTLC is fast (5 min), allows simultaneous separation and can be carried out with the same carrier materials as HPLC. Silica gel and chemically bonded silica gel sorbents are used predominantly in HPTLC other stationary phases are cellulose-based [393]. Separation mechanisms are either NPC (normal-phase chromatography), RPC (reversed-phase chromatography) or IEC (ion-exchange chromatography). RPC on hydrophobic layers is not as widely used in TLC as it is in column chromatography. The resolution capabilities of TLC using silica gel absorbent as compared to C S reversed-phase absorbent have been compared for 18 commercially available plasticisers, and 52 amine and 36 phenolic AOs [394]. [Pg.221]

RP-HPLC Reversed-phase high performance liquid chromatography TFA Trifluoroacetic acid... [Pg.291]

Our initial attempts to separate the Cj and Dj isomers of 5 (G = 1) used octadecyl polysiloxane (ODS) as high performance liquid chromatography (HPLC) stationary phase and mixtures of acetonitrile/HjO or methanol/HjO as mobile phases. Under these classical reverse-phase conditions, the resulting efficiencies were extremely poor because of the low solubility of 5 (G = 1) in both mobile phases. By contrast, mixed mobile phases which contained acetonitrile (ACN) with some percentages of a cosolvent such as tetrahydrofuran (THF) substantially improved... [Pg.47]

RP-HPLC reversed-phase high-pressure thin-layer chromatography... [Pg.125]

PGL-I, homogeneous by thin-layer chromatography, through reverse-phase HPLC showed heterogeneity based on fatty acid content, and PD-MS analysis demonstrated the existence of the C3o,C3o mycocerosate glycolipid, the C32,C32 species, and the species.27 Two other minor phenolic glycolipids isolated from M. [Pg.201]

Fig. 3.11 shows chromatograms of 2,3-dihydroxybenzoate and 2,5-dihyroxybenzoate separated by HPLC reversed-phase chromatography fitted with electrochemical detection (Halliwell et al., 1988). HPLC is carried out on a Spherisorb 50DS column (25 cm x 4.6 mm) applying 97.2% (v/v) 30 mM sodium citrate/2.7 mM acetate... [Pg.84]

As opposed to normal-phase HPLC, reversed-phase chromatography employs mainly dispersive forces (hydrophobic or van der Waals interactions). The polarities of mobile and stationary phases are reversed, such that the surface of the stationary phase in RP HPLC is hydrophobic and mobile phase is polar, where mainly water-based solutions are employed. [Pg.11]

RP-HPLC Reverse-phase high-performance hquid chromatography... [Pg.20]

See other pages where Chromatography HPLC, reversed phase is mentioned: [Pg.142]    [Pg.386]    [Pg.13]    [Pg.98]    [Pg.142]    [Pg.386]    [Pg.13]    [Pg.98]    [Pg.126]    [Pg.218]    [Pg.67]    [Pg.88]    [Pg.11]    [Pg.538]    [Pg.520]    [Pg.348]    [Pg.500]    [Pg.126]    [Pg.163]    [Pg.46]    [Pg.443]    [Pg.103]    [Pg.95]    [Pg.576]    [Pg.467]    [Pg.49]    [Pg.289]    [Pg.114]    [Pg.241]   
See also in sourсe #XX -- [ Pg.62 ]



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