Chromatography, apparatus for continuous

An apparatus for continuous-flow solution-phase reactions has been described (68) combining a flow reactor with a solvent extraction, continuous evaporation, and liquid chromatography system. A scheme for the use of this apparatus in dinucleotide synthesis has been proposed (46), but applications to the preparation of longer oligonucleotides have not been reported. 

The apparatus used for continuous-flow paper electrophoresis can be used for separation with an elution rather than a continuous-flow method. The sample is spotted at the top of the paper and only the buffer solution is continuously added. If the electrophoresis is stopped before the sample components drip from the bottom of the paper, the technique combines the separating ability of descending paper chromatography (separation occurring vertically) with that of electrophoresis (separation occurring horizontally) to yield spots simultaneously developed in two dimensions. 

T. (1989) Automated micro stopped-flow/continuous-flow apparatus for serial measurement of enzyme reactions and its application as a real-time analyser for column chromatography. Anal. Chim. Acta, 220, 13—21. doi 10.1016/80003-2670(00)80246-9... 

Continuous chromatography in the packed annular space between the walls of two concentric cylinders can be done by rotating the assembly about its longitudinal axis (1, 2, 3). Rotation transforms the temporal separation that would be obtained under fixed, pulsed operation into a spatial separation that permits continuous operation. It has recently been shown that continuous reaction chromatography can be done in similar apparatus (4, Jj). This not only provides a means of carrying out chemical reaction and separation simultaneously in one unit, but for A B + C the product separation suppresses the rate of the back reaction and provides a means of enhancing the reaction yield. Yield enhancement in pulsed column chromatography has been demonstrated (6, 8). Yields of... 

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