Chromatographic size exclusion chromatography

Fig. 1. Classification of chromatographic systems where gsc is gas—soHd chromatography glc, gas—Hquid chromatography sec, size-exclusion chromatography Isc, Hquid—soHd chromatography Uc, Hquid—Hquid chromatography iec, ion-exchange chromatography tic, thin-layer chromatography ...

The effects of various pore-size distributions, including Gaussian, rectangular distributions, and continuous power-law, coupled with an assumption of cylindrical pores and mass transfer resistance on chromatographic behavior, have been developed by Goto and McCoy [139]. This study utilized the method of moments to determine the effects of the various distributions on mean retention and band spreading in size exclusion chromatography. 

Size exclusion chromatography (SEC) separates molecules of a polymer sample on the basis of hydrodynamic volume. When the chromatograph is equipped only with a concentration-sensitive detector, i.e. conventional SEC, a molecular weight distribution (MWD) can be obtained from the chromatogram only through use of a calibration function relating molecular weight and elution volume V (2). 

Wu, C. S., Senak, L., and Malawer, E. G., Size exclusion chromatography of poly(methyl vinyl ether-co-maleic anhydride) (PMVEMA). I. The chromatographic method, J. Liq.Chromatogr., 12, 2901, 1989. 

Jackson, C. and Yau, W. W., Computer simulation study of multidetector size-exclusion chromatography. Flory-Schulz molecular weight distribution, in Chromatographic Characterization of Polymers, Hyphenated and Multidimensional Techniques, Provder, T., Barth, H. G., and Urban, M. W., Eds., American Chemical Society, Washington, D.C., 1995, chap. 6. 

Standard Test Method for Molecular Weight Averages and Molecular Weight Distribution of Certain Polymers by Liquid Size-Exclusion Chromatography (Gel Permeation Chromatograph -GPC) Using Universal Calibration" ASTM D 3593 80, Annual Book of ASTM Standards, ASTM, Philadelphia, PA, revised annually. 

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