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Chromatographic processes size exclusion chromatography

Size-exclusion chromatography is basically different from all other chromatographic methods in that a simple molecule size classification process rather than any interaction phenomena forms the basis of separation. [Pg.231]

The practical use of chromatographic and electrophoretic separations in the biotechnology industry will be aided by correlations capable of relating bench scale results to process scale conditions. Published physical and chemical property data on chromatographic systems for engineering calculation purposes currently appears to be limited. Hence, an effort was made in this chapter to present equations which can be used with physical parameters for which values have been reported or which are readily determined on a bench scale. Since size exclusion chromatography is important in fractionation of proteins, the rationale and equations were presented for estimating ... [Pg.146]


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