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Chromatographic modes reversed phase

HPLC Assay of an ethyl alcohol-acetic acid extract against a Reference Standard material (chromatographic conditions reversed-phase mode Cjo column methanol—water—acetic acid, 50 49 1 (v/v), mobile phase detection at 254 nm [79]) and (c) extraction of tablets with methanol or ethanol and UV measurements at 260 or 262 nm. Linearity in the 3—15 Ug/ml and standard errors of less than 2% were reported [65,109,110]. A similar procedure is used for the determination of the Content Uniformity of the tablets [79].. ... [Pg.295]

Chromatographic Mode Normal Phase (Silica, Florisil , Alumina, Diol, NH2, etc.) Reverse Phase (C18, CN, etc.) Ion Exchange (NH2, Anion Exchange, Cation Exchange, etc.)... [Pg.270]

Unlike the more popular reversed-phase chromatographic mode, normal-phase chromatography employs polar stationary phases, and retention is modulated mainly with nonpolar eluents. The stationary phase is either (a) an inorganic adsorbent like silica or alumina or (b) a polar bonded phase containing cyano, diol, or amino functional groups on a silica support. Tlie mobile phase is usually a nonaqueous mixture of organic solvents. As the polarity of the mobile phase decreases, retention in normal-phase chromatography... [Pg.241]

By Chromatographic Modes Normal-phase (NPC), reversed-phase (RPC), ion-exchange (IEC), size-exclusion (SEC)... [Pg.48]

Macrocyclic glycopeptides. The first of these CSPs - based on the cavity of the antibiotic vancomycin bound to silica - was introduced by Armstrong [25]. Two more polycyclic antibiotics teicoplanin and ristocetin A, were also demonstrated later. These selectors are quite rugged and operate adequately in both normal-phase and reversed-phase chromatographic modes. However, only a limited number of such selectors is available, and their cost is rather high. [Pg.58]

Tswett s initial column liquid chromatography method was developed, tested, and applied in two parallel modes, liquid-solid adsorption and liquid-liquid partition. Adsorption ehromatography, based on a purely physical principle of adsorption, eonsiderably outperformed its partition counterpart with mechanically coated stationary phases to become the most important liquid chromatographic method. This remains true today in thin-layer chromatography (TLC), for which silica gel is by far the major stationary phase. In column chromatography, however, reversed-phase liquid ehromatography using chemically bonded stationary phases is the most popular method. [Pg.3]

From the general framework of the Snyder and Soczewinski model of the linear adsorption TLC, two very simple relationships were derived, which proved extremely useful for rapid prediction of solute retention in the thin-layer chromatographic systems employing binary mobile phases. One of them (known as the Soczewinski equation) proved successful in the case of the adsorption and the normal phase TLC modes. Another (known as the Snyder equation) proved similarly successful in the case of the reversed-phase TLC mode. [Pg.18]

The PRISMA model was developed by Nyiredy for solvent optimization in TLC and HPLC [142,168-171]. The PRISMA model consists of three parts the selection of the chromatographic system, optimization of the selected mobile phases, and the selection of the development method. Since silica is the most widely used stationary phase in TLC, the optimization procedure always starts with this phase, although the method is equally applicable to all chemically bonded phases in the normal or reversed-phase mode. For the selection of suitable solvents the first experiments are carried out on TLC plates in unsaturated... [Pg.866]

If simple sample pretreatment procedures are insufficient to simplify the complex matrix often observed in process mixtures, multidimensional chromatography may be required. Manual fraction collection from one separation mode and re-injection into a second mode are impractical, so automatic collection and reinjection techniques are preferred. For example, a programmed temperature vaporizer has been used to transfer fractions of sterols such as cholesterol and stigmasterol from a reversed phase HPLC system to a gas chromatographic system.11 Interfacing gel permeation HPLC and supercritical fluid chromatography is useful for nonvolatile or thermally unstable analytes and was demonstrated to be extremely useful for separation of compounds such as pentaerythritol tetrastearate and a C36 hydrocarbon standard.12... [Pg.91]

Thick-layer silica gel chromatography can also be employed [7], although most separations are now accomplished by high-performance liquid chromatography. Resolution of complex mixtures often requires both normal and reverse phase modes [19]. A further dimension is added, when bioactivity is correlated with spectroscopically-monitored chromatographic profiles. [Pg.44]

A combination of different modes of chromatography can provide excellent resolution for those components that chromatograph poorly with a single mode [108,109]. The chromatographic separation of baclofen (I) from its potential transformation product (II) with dual mode chromatography entailing ion-pair reversed-phase chromatography and... [Pg.557]


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Chromatographic phases

Chromatographic separation, modes reversed phase chromatography

Reversed phase chromatograph

Reversed-phase HPLC chromatographic modes

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