Chiral monoterpenoids enantioselective analysis

Chiral monoterpenoids in plants-enantioselective chromatographic analysis, and their bioactivity... 

CHIRAL MONOTERPENOIDS IN PLANTS -ENANTIOSELECTIVE CHROMATOGRAPHIC ANALYSIS, AND THEIR BIOACTIVITY... 

After successful application of enantioselective GC to the analysis of enantiomeric composition of monoterpenoids in many essential oils (e.g., Werkhoff et al., 1993 Bicchi et al., 1995 and references cited therein), the studies have been extended to the sesquiterpene fraction. Standard mixtures of known enantiomeric composition were prepared by isolation of individual enantiomers from numerous essential oils by preparative GC and by preparative enantioselective GC. A gas chromatographic separation of a series of isolated or prepared sesquiterpene hydrocarbon enantiomers, showing the separation of 12 commonly occurring sesquiterpene hydrocarbons on a 2,6-methyl-3-pentyl-P-cyclodextrin capillary column has been presented by Konig et al. (1995). Further investigations on sesquiterpenes have been published by Konig et al. (1994). However, due to the complexity of the sesquiterpene pattern in many essential oils, it is often impossible to perform directly an enantioselective analysis by coinjection with standard samples on a capillary column with a chiral stationary phase alone. Therefore, in many cases two-dimensional GC had to be performed. 

C. Askari, P. Kreis, A. Mosandl and H.-G. Schmarr. Chiral Compounds of Essential Oils Vll Quality Evaluation of Mentha Oils, using enantioselective CGC-Analysis of Monoterpenoid Constituents. Arch. Pharm., 325, 35-39 (1992). 

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