Chinese Hamster Ovarian

The different furanones 104 were tested for their potency as inhibitors of PGE2 production both in transfected Chinese hamster ovarian (CHO) cells expressing human COX-2 and in human whole blood. Compound 104r proved to be an orally active and selective COX-2 inhibitor that is devoid of the ulcerogenic effect at >100 times the dose for antiinflammatory, analgesic, and antipyretic effects (99BMC3187). 

Rouiller DG, Sharon N, McElduff A, Podskalny JM, Gorden P 1986 Lectins as probes of insulin receptor carbohydrate composition studies in glycosylation mutants of Chinese hamster ovarian cells with altered insulin binding. Endocrinology 118 1159-1165... 

The genetic manipulation of animal cells allows the production of therapeutic proteins in animal cell culture systems. Mammalian cells such as Chinese hamster ovarian cells and baby hamster kidney cells are commonly used. These mammalian hosts produce recombinant proteins that have almost identical properties to those made by human cells. However, the use of mammalian cells does have disadvantages. As noted earlier, they are expensive to use. This is influenced by their more complex nutritional requirements, their slower growth, and their increased susceptibility to physical damage (Walsh, 2003). 

HEK293 cells are perfectly used to express and study liver specific uptake transporters like organic anion transporting polypeptides (OATPs). One should consider the endogenous expression of OATs if using HEK293 cells for the kidney specific uptake transporter. In these cases transfection of CHO (Chinese hamster ovarian) cells might be preferred. 

Livingston GK, Rom WN, Morris MV. 1980. Asbestos-induced sister chromatid exchanges in cultured Chinese hamster ovarian fibroblast cells. J Environ Pathol Toxicol 3 373-382. 

Poly(lysine-C( -phthalamide) has been characterized with side chains of poly(ethylene glycol) (PEG) or Jeffamine M-1000 , a hydrophilic PEG analogue [33-35]. These polymers show minimal cytotoxicity when tested against HeLa and Chinese hamster ovarian cells, where viability is at least 60% at polymer concentrations of 2 mg/ml [34]. Poly(lysine-C( -phthalamide) is generally nonhemolytic at physiological pH, but the incorporation of... 

When expressed by transfected tumour cells, IL-10 decreased the tumorigenicity of Chinese hamster ovarian carcinoma cells (Richter et al. 1993), CL8-1 cells (Suzuki et al. 1995), TS/A cells (Giova-relli et al. 1995) and B16-F1 melanoma cells (GfiRARD et al. 1996). 

A comparison of the cell-surface glycoproteins from wild-type, Chinese-hamster ovarian cells with those resistant to the lectin from Phaseolus vulgaris suggested that they differ in the carbohydrate moiety of the membrane glycoproteins. ... 

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