Chicken monosialogangliosides

Comparison of glycosphingolipids from human and chicken skeletal muscle. The elution of gangliosides from DEAE-Sephadex A 50 column with these 0.01, 0.02 and 0.2 M sodium acetate concentrations separated the gangliosides into mono-, di- and poly-sialo- fractions. The gangliosides of human muscle are shown in Fig. 1A. The monosialogangliosides GM3, GM2 and GM1 were eluted with 0.01 M sodium acetate in methanol (lane 2), GD3 and GDla with the 0.02 M solvent (lane 4) and others with 0.2 M acetate... 

Figure 2. Elution pattern from Bio-Sil A column of monosialogangliosides prepared from chicken pectoral muscle. Column was eluted with C M HtO (130 70 12, v/v) and changed to C M HtO (120 70 14, v/v) at the arrow.

FATTY ACIDS AND LONG CHAIN BASES OF CHICKEN MUSCLE MONOSIALOGANGLIOSIDES... 

Kaufman, B., Basu, S., and Roseman, S., 1968, Enzymatic synthesis of disialogangliosides from monosialogangliosides by sialyltransferases from embryonic chicken brain, J. Biol. Chem. 243 5804-5806. 

---