Chicken glycosphingolipids from

Comparison of glycosphingolipids from human and chicken skeletal muscle. The elution of gangliosides from DEAE-Sephadex A 50 column with these 0.01, 0.02 and 0.2 M sodium acetate concentrations separated the gangliosides into mono-, di- and poly-sialo- fractions. The gangliosides of human muscle are shown in Fig. 1A. The monosialogangliosides GM3, GM2 and GM1 were eluted with 0.01 M sodium acetate in methanol (lane 2), GD3 and GDla with the 0.02 M solvent (lane 4) and others with 0.2 M acetate... 

The results presented here show that the glycosphingolipids of chicken pectoral muscle differ from those of human skeletal and cardiac muscles. In addition, we are presenting the structures of two glucosamine-containing gangliosides which were characterized by enzymatic hydrolysis and methylation studies. 

A sulphated neolactotetraosylceramide isolated from hog gastric mucosa was characterized as (19) by conventional procedures. A unique D-gluco-sphingolipid pattern which has not been previously encountered in any type of tissue or cell has been seen in chicken egg-yolk. The structures were determined and the major ones were found to be NeupiVAc -> o-Galp - ceramide, NeupAAc lactosyl ceramide, and NeupAAc NeupAAc -> lactosyl -> ceramide. The only other neutral glycosphingolipid was D-galactosylceramide. 

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