Chicken column chromatography

Fig. 16. Separation of cytochrome c (1), myoglobin (2), and chicken egg albumin (3) by re-versed-phase chromatography on a monolithic poly(styrene-co-divinylbenzene) column at flow rates of a 5 ml/min b 25 ml/min. (Reprinted with permission from [53]. Copyright 1996 American Chemical Society). Conditions column 50 mmx8 mm i.d., mobile phase linear gradient from 20 to 60% acetonitrile in water... 

Fig. 4 The total ion chromatography (TIC) of the separation of a tryptic digest of chicken ovalbumin with a sample injection amount of 12 pmol corresponding to the original protein [52]. Column length, 6 cm. Conditions 20 min, 0-40% acetonitrile gradient 1000 V applied voltage with a 40-bar supplementary pressure. (From Ref. 52 reproduced with permission of the authors and the American Chemical Society.)...

Adam et ai, 1995 Childs and Mak, 1993 Katoch and Moreland, 1995). In chicken gizzard and rat aorta, immunoprecipitated MAPK exhibits phosphotransferase activity toward myelin basic protein and purified caldesmon. When crudely extracted from porcine carotid arteries, MAPK exhibits phosphotransferase activity that has been quantitated using a peptide substrate (APRTPGGRR). To determine if the peptide kinase activity detected in crude tissue extracts is specific for MAPK (the peptide may detect other protein kinases), proteins in the extracts have been separated on a Mono-Q fast-performance liquid chromatography column as shown in Fig. 2, and further characterized. Only two peaks of phosphotransferase activity... 

Figure 7.1 Gas chromatography-mass spectrometry chromatogram of hydrocarbons from chicken meat. Chromatograph HP 5890/5970 GC-MS system, DB-5 column, 12.5 m x 0.2 mm inner diameter, 0.33 pm film thickness carrier gas = helium. 

Figure 7.4 Separation of 2-dodecylcyclobutanone (DCS) in irradiated chicken meat (labelled with dansylhydrazine). (a) Without thindayer chromatography (TLC) (b) after TLC separation (c) after co-injection of reference material. Chromatographic conditions NucleosiF 100 packing RP-18 coating 3 pm film thickness 250 mm x 4.6 mm inner diameter column solvent system, methanol/water (90 10 vol./vol).

The amount of 5-phospho-a D-ribosyl pyrophosphate (PRPP) in chicken liver was measured using the procedure of LaLanne and Henderson (9). The soluble purine base levels were determined using freeze-clamped liver that was extracted with perchloric acid, centrifuged and then boiled to hydrolyze the soluble nucleotides to the free bases. The bases were separated and quantitated on a Partisil pressure liquid chromatography column (Whatman). 

Figure 3 HPSEC of a Triton X-100 extract ot purified Sendai and Newcastle disease virions. Virus was grown in 10-day-old embryonated chicken eggs and purified and extracted as described [5]. A TSK 4000SW (600 x 7.5 mm ID) column was eiuted with 50 mM sodium phosphate, pH 6.5, containing 0.1% SDS. Samples were boiled for 2 min in 4% SDS prior to chromatography. The flow rate was 1 mL/min and the absorbance was monitored at 280 nm. 1, Tetramer of the hemagglutinin neuraminidase (HN) protein of Sendai virus 2, dimer of HN 3, Sendai virus fusion protein F , Triton X-100.

FIGURE 10.17 Detection of thiocompounds in various samples by High-performance anion-exchange chromatography with integrated pulsed amperometric detection (HPLC-IPAD). Samples were (A) grapefruit juice, (B) watermelon, and (C) chicken liver. Condition Ci8, 1-mm SepStik column 99.6% 100 mM phosphate buffer (pH 3)/0.4% acetonitrile 65 p,L/min 0.5 p,L injection volume and detection, IPAD waveform in Table 10.3. Peaks a, GSH b, methionine and c, GSSG. (Reprinted from LaCourse, W.R. and Owens, G.S., Anal. Chim. Acta, 307, 301, 1995. With permission.)... 

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