Chemiluminescence detection system

We also examined the preservation stability of the photoimmobilized antibodies. Although the reactivity of the antibodies dropped away over a period of 10 days when they were stored at room temperature, it was maintained for about 2 months when stored at 4°C. This result is acceptable in terms of commercial viability, though further increase in stability would be preferable. We next examined the sensitivity for the immunochips. An immu-nochip usually has a two-dimensional surface, so the detection limit for antigens can be estimated from the amount of immobilized antibodies that are present. It was difficult to increase the sensitivity of a detection system which uses a photoluminescence probe. However, we succeeded in obtaining higher sensitivity for an immunochip in which we adopted a chemiluminescence detection system using an enzyme reaction. 

In Far-Western blotting the membrane is probed with another protein to detect specific protein-protein interactions (24). The reaction can be revealed using biotinylated or GST-tagged bait or probe protein followed by a streptavidin-HRP or an anti-GST-HRP chemiluminescent detection system, respectively. 

Richardson et al. [117] have applied gas chromatography with a chemiluminescence detection system to the determination of microgram levels of nitrosamines (N-nitrosodimethylamine, A-nitrosodiethylamine, A-nitroso mo rpho 1 i nc and A-... 

A similar technique that bypasses the electrophoretic separation step is known as dot blotting. A protein sample to he analyzed is applied to a membrane surface as a small dot and dried. The membrane is then exposed to a labeled antibody specific for the test antigen contained in the dotted protein mixture. After washing, bound labeled antibody is detected with a photometric or chemiluminescent detection system. 

Imai K, Nishitani A, Tsukamoto Y, Wang WH, Kanda S, Hayakawa K, Miyazaki M. Studies on the effects of imidazole on the peroxyoxalate chemiluminescence detection system for high performance liquid chromatography. Biomed Chromatogr 1990 4 100-204. 

DETERMINATION OF PARTICLE-ASSOCIATED NITRO-PAH USING HPLC/CHEMILUMINESCENCE DETECTION SYSTEM... 

In this study, the concentrations of several kinds of nitro-PAH, such as 1-nitropyrene (1-NP), 2-nitropyrene (2-NP), 2-nitrofluoranthene (2-NF), and 2-NTP, in the soluble organic fraction of airborne particles were determined by a column switching HPLC-chemiluminescence detection system in order to clarify the occurrence and behaviour of 2-NTP in the atmosphere. 

Imai K. Chemiluminescence detection system for high-performance liquid chromatography. Methods Enzymol 1986 133 435-49. 

Nozaki O, Kawamoto H. Reactivation of horseradish peroxidase with imiaazole for continuous determination of hydrogen peroxide using a micro-flow injection -chemiluminescence detection system. Luminescence 2003 18 203-6. 

Marzocchi E, Grilli S, Della Ciana L, Mirasoli M, Prodi L, Roda A. Chemiluminescent detection systems of horseradish peroxidase employing nucleophilic acylation catalysts. Anal Biochem, accepted for publication. 

Baeyens, W., Bruggeman, J., DeWaele, C., Lin, B., and Imai, K., Optimization of an HPLC peroxylate chemiluminescence detection system for some dansyl amino acids. J. Biolumin. Chemilumin. 5, 13-23 (1990). 

Imai, K., Chemiluminescence detection in flow systems. Biomed. Chromatog. 4, R1 (1990). Imai, K., Nishitan, A., Tuskamoto, Y., Wang, W. H., Kanda, S., Hayakawa, K., and Miyazaki, M., Studies on the effects of imidazole on the peroxyoxalate chemiluminescence detection system for high performance liquid chromatography. Biomed. Chromatog. 4,100-104 (1990). Ireland, D. C., and Samuel, D., Enhanced chemiluminescence ELISA for the detection of antibody to hepatitis B virus surface antigen. J. Biolumin. Chemilumin. 4, 159-163 (1989). 

K14. Kobayashi, S., Sekino, J., Honda, K., and Imai, K., Application of high performance liquid chromatography with a chemiluminescence detection system to determine catecholamines in urine. Ana/. Biochem. 112, 99-104 (1981). 

Tsukagoshi, K., Nakahama, K., and Nakajima, R., Direct detection of biomolecules in a capillary electrophoresis-chemiluminescence detection system. Ana/. Chem., 76, 4410, 2004. 

Both flow-based and array-based chemiluminescence detection systems have been incorporated into micro total analysis systems (pTAS) and other microfluidic devices in conjunction with the myriad recent advances and innovations in microfluidics technology such as device fabrication, sample and reagent preparation and handling, and analyte selectivity, as described throughout this Encyclopedia. 

Biotinylated PCR products For purposes of nonradioactive detection, a single biotin molecule is incorporated into the 5 end of one or both of the PCR primer pairs. The biotinylated PCR products are used in a chemiluminescent detection system as described below. Attachment of biotin molecules to oligonucleotides has been described in detail (12). For an alternative method of biotinylating PCR products, see Note 3. 

Anastos N, Lewis SW, Barnett NW, Sims DN (2006) The determination of psilocin and psilocybin in hallucinogenic mushrooms by HPLC utilizing a dual reagent acidic potassium permanganate and tris (2,2-bipyridyl) ruthenium (11) chemiluminescence detection system. J Forensic Sci 51 45-51... 

Kimoto, K. Gohda, R. Murayama, K. Santa, T. P ikushima, T. Homma, H. Imai, K. Sensitive detection of near-infrared fluorescent dyes using high-performance liquid chromatography with peroxalate chemiluminescence detection system, Biomed.Chromatogr., 1996,10, 189-190. 

Richardson et al. [117] have applied gas chromatography with a chemiluminescence detection system to the determination of microgram levels of nitrosamines (N-nitrosodimethylamine, N-nitrosodiethylamine, N-nitrosomorpholine and N-nitrosodiethanolamine, N-nitrosopyrrolid-ine, N-nitrosopiperidine and N-nitroso-5-methyl-l,3-oxazolidine) in potable water supplies. Nitrosamines may be removed from aqueous media by solvent extraction and subsequently concentrated by evaporation of the solvent, in order to detect levels as low as O.Olpg L... 

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