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Chemical genetics applications

Campagna-Slater, V. and Schapira, M. 2009. Evaluation of virtual screening as a tool for chemical genetic applications. ]. Chem. Inf. Model. 49 2082-2091. [Pg.198]

The application of forward chemical genetics to studies of translation provides an opportunity to identify small molecules that inhibit or stimulate this process without any underlying assumptions as to which step is most amenable to targeting by the chemical libraries under consideration. The opportunity exists to identify novel factors involved in translation, unravel new activities of known translation initiation factors, or characterize shortlived intermediates that are frozen by the small molecule inhibitor. We have undertaken a forward chemical genetic approach to identify small molecules that inhibit or stimulate translation in extracts prepared from Krebs-2 ascites cells (Novae et al., 2004). These screens have led to the identification of several novel inhibitors of translation initiation and elongation (Bordeleau et al., 2005, 2006 Robert et al., 2006a,b). [Pg.315]

Effective P13K/PKB pathway interruption has also been reported with a series of compoimds whose mechanism of action is still unknown. The three examples reviewed in this section also illustrate the potential application and challenges that chemical genetics may face in this area of drug discovery. [Pg.197]

The progress of reverse chemical genetics research is influenced by the efficiency of generation of active recombinant proteins. In recent years, numerous systems for the expression of the recombinant proteins have been developed. Of these, the baculovirus system is considered to be the most efficient. Typically in the baculovirus system, an insect cell line (for example, Sf9) is used as a host for the expression of recombinant proteins. In the present report, we describe the novel application of Kaiko as a host in the baculovirus system for the expression of recombinant... [Pg.109]

To date, complex proteins with biological activity (6), for use in X-ray crystal structure analysis (7) and ELISA systems (8), and for the development of animal drugs (9) have successfully been produced by using this system. Therefore, the Kaiko-baculovirus protein production system has broad applicability across the field of reverse chemical genetics for the analysis of protein function on the basis of interactions with chemical compounds. [Pg.118]

This concludes a discussion of exactly solvable second-order processes. As one can see, only a very few second-order cases can be solved exactly for their time dependence. The more complicated reversible reactions such as 2Apt C seem to lead to very complicated generating functions in terms of Lame functions and the like. This shows that even for reasonably simple second- and third-order reactions, approximate techniques are needed. This is not only true in chemical kinetic applications, but in others as well, such as population and genetic models. The actual models in these fields are beyond the scope of this review, but the mathematical problems are very similar. Reference 62 contains a discussion of many of these models. A few of the approximations that have been tried are discussed in Ref. 67. It should also be pointed out at this point that the application of these intuitive methods to chemical kinetics have never been justified at a fundamental level and so the results, although intuitively plausible, can be reasonably subject to doubt. [Pg.165]

Recently, new comprehensive approaches have been developed that use compound libraries for screens in whole living cells using specific phenotype readouts. Some of the applications of this type of approach have been called chemical genetics or chemogenomics [9-27]. In many instances it is probably more accurate to call... [Pg.313]

The first chapter of the book provides an introduction to MOO with a realistic application, namely, the alkylation process optimization for two objectives. The second chapter reviews nearly 100 chemical engineering applications of MOO since the year 2000 to mid-2007. The next 5 chapters are on the selected MOO techniques they include (1) review of multi-objective evolutionary algorithms in the context of chemical engineering, (2) multi-objective genetic algorithm and simulated... [Pg.441]

LIGAND AND INHIBITOR DISCOVERY USING CHEMICAL ARRAYS AND ITS APPLICATION TO CHEMICAL GENETIC STUDY... [Pg.72]


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See also in sourсe #XX -- [ Pg.59 , Pg.72 , Pg.75 ]




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