Cerebellum basket cells

Both anandamide and 2-AG are inactivated by enzymatic hydrolysis (Goparaju et al. 1998). Fatty acid amide hydrolase (FAAH) is an enzyme that catalyses their hydrolysis. High concentrations of FAAH were found in the cerebellum, hippocampus and neocortex of rat brain, which are also rich in cannabinoid receptors. Further, there is a complementary pattern of distribution of FAAH and the CBl receptor. For example, in the cerebellum, FAAH is found in the cell bodies of Purkinje cells and the CBl receptor is found in the axons of granule cells and basket cells, which are presynaptic to Purkinje cells. 2-AG may also be inactivated by direct esterification into membrane phospholipids. Cannabinoid Receptors... 

Glutamate is a key neurotransmitter in the cerebellum mossy fibres onto granule cells parallel fibres onto Purkinje and stellate/basket cells, climbing fibres onto Purkinje cells, and also mossy fibre and climbing fibre inputs onto Golgi cells all use glutamate (Fig. 19) (Voogd... 

Purkinje cells. The recurrent collaterals extend into the molecular layer where they contact basket cells (O Donoghue et al., 1989). The whole collateral arborization is oriented perpendicular to the long axis of the folia, i.e. in the same plane as the dendritic tree of the Purkinje cell. In the cat it measures 300-700 //m in the sagittal and 100 00 m in the transverse direction (Bishop, 1988). The width of the arborization and its penetration in the molecular and granular layers varies for different parts of the cerebellum. Recurrent collaterals of Purkinje cell axons are constituents of the infra- and supraganglionic plexus. The main Purkinje cell axon enters and traverses the white matter to terminate on cells of the cerebellar or the vestibular nuclei. 

Polyclonal antibodies against parvalbumin stain all Purkinje cells and stellate and basket cells in the molecular layer of rat and avian cerebellum (Figs 31B and 32) (Celio and Heizmann, 1981 Heizmann, 1984 Braun et al., 1986 Endo et al., 1985 Schneeberger et al., 1985 Seto-Oshima et al., 1983 Rogers, 1989 Kosaka et al., 1993). The localization of parvalbumin in Purkinje, stellate and basket cells was confirmed in the rat with non-radioactive in situ hybridization (Kadowaki et al., 1993). Parvalbumin... 

Fig. 36. Immunocytochemical staining patterns of two monoclonal anti-bodies directed against non-phosphorylated and phosphorylated neurofilaments were studied in the cerebellum of developing normal rats. A. Non-phosphorylated neurofilaments on postnatal day 11. B. Day 21. Basket cell axons form a characteristic brush-like plexus around the initial segment of the Purkinje cell axon. C. Phosphorylated neurofilaments on postnatal day 13, D. Postnatal day 21. Stained filaments are restricted to Purkinje cell and basket cell axons and are absent from the Purkinje cell cytoplasm. Calibration bars in A and C 30/tm, in B and D 10/tm. Marc et al. (1986).

Fig. 71. Bright-field photomicrographs showing cellular distribution of mRNAs of al, a6, 2, yS3, y2 and S GABAa receptor subunits in the cerebellum of the rat. Arrows indicate examples of labelled stellate/basket cells arrowheads delineate Purkinje cells. Gr, granule cells Mol, molecular layer P, Purkinje cells. Scale bar = 50 //m. Laurie et al. (1992).

CYP2D in rat cerebellum, the most intensely immu-noreactive region on Western blots, is strong in both Purkinje cells and stellate and basket cells of... 

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