Centrifugal ultrafiltration

Compared to non-membrane processes (chromatography, dialysis, solvent extraction, or centrifugation), ultrafiltration is far gentler to the molecules being processed, does not require... 

MS multiple compounds in solution target in solution ligands and subjected to centrifugal ultrafiltration binding compounds separated from non-binders that are washed to waste ligands bound to target are eluted by acidification and detected by MS Zweigenbaum, Henion, 1997)... 

Georgiou HM, Rice GE, Baker MS. Proteomic analysis of human plasma Failure of centrifugal ultrafiltration to remove albumin and other high molecular weight proteins. Proteomics 2001 1(12) 1503-1506. 

Figure 3-4. Ultrafiltration by centrifugation. Ultrafiltration by centrifugation is particularly well suited for relatively small volumes. The solution is introduced into the upper chamber of the centrifugation cartridge, and on centrifu-...

The DNA can be conveniently concentrated using a centrifugational ultrafiltration device (10,000MW cut-off), such as a Tfentricon (fixMn Amicon). 

A modular pilot size plant involving coagulation/flocculation, centrifugation, ultrafiltration, and sorption processes was designed and constmeted by Benito et al. [13] for the treatment of oily wastewaters. Empirical equations developed by Shaalan [65] predict the impact of water contaminants on flux decline. These formulae enable decision-making concerning a suitable water pretreatment scheme and also selection of the most appropriate cleaning cycle. 

Hammond GL, Nisker JA, Jones LA, Siiteri PK. Estimation of the percentage of free steroid in undiluted serum by centrifugal ultrafiltration-dialysis. J Biol Chem 1980 255 5023-6. 

T vo approaches can be adopted for dealing with the overlap problem. Firstly, simple sample preparation such as centrifugal ultrafiltration can be used to remove the macromolecules. This results in a spectrum of all the nonprotein-bound metabolites contributing to the spectrum. Alternatively, to avoid s unple manipulation, a spectral editing technique can be applied. It has... 

Emphasis is on rational theory and its consequences, with the purpose of showing the underlying unity of PCH, in which diverse phenomena can be described in physically and mathematically similar ways. The magic of this unity is shown in the similar manner in which solutes concentrate in a flow containing chemically reacting surfaces, reverse osmosis membranes, and electrodialysis membranes or the similarity of particle motions in sedimentation, centrifugation, ultrafiltration, and electrophoresis. Experimental results, numerical solutions, and reference to topics not covered are noted where they serve to illustrate a concept, result, or limitation of what has been presented. Empiricism is not eschewed, but only limited use is made of it and then only when it contributes to a better understanding of an idea or phenomenon. 

Goto et al. " described a simple, rapid, and simultaneous analysis method for oxytetracycline, tetracycline, chlortetracycline, penicillin G, ampicillin, and nafcillin in meat using electrospray ionization tandem mass spectrometry. The samples were homogenized with water followed by a centrifugal ultrafiltration after addition of internal standards (demeclocycline, penicillin G-ds, ampicillin-fi 5, and... 

Turano E, Curdo S, De Paola M G, Calabrb V, lorio G (2002), An integrated centrifugation-ultrafiltration system in the treatment of olive mill wastewater , / Membrane Sci., 209(2), 519-531. 

Nanocep centrifugal ultrafiltration devices, MW 3 kDa (Pall Corporation). 

Concentrate cell lysates by centrifugation for 15 min at 16,000 at 4°C with Nanocep centrifugal ultrafiltration devices (see Note 7). 

The in-vitro drug release kinetic profile of clofibride from a nanoemulsion was determined using two different kinetic techniques the bulk equilibrium reverse dialysis sac technique, and the centrifugal ultrafiltration technique at low pressure. The former technique was shown to be inadequate for in-vitro kinetic comparison purposes as a result of drug diffusion limitations through the dialysis membrane. The latter technique yielded rapid in-vitro release profiles of clofibride from the nanoemulsion under perfect sink conditions. The kinetic results clearly exclude the use of a nanoemulsion as a colloidal controlled release delivery systems for any administration route where perfect sink conditions should prevail. ... 

To avoid the above problems we routinely use an extraction method in which DNA fragments become bound to hyroxyapatite powder (9). This is handled in the form of a centriftiged chromatography column for the purposes of washing with various buffers and final elution of pure DNA in 0.5 M potassium phosphate. This DNA is concentrated and desalted in a centrifugal ultrafiltration device (Centricon-10 from Amicon). Many commercial kits are available for the isolation of pure DNA from biological samples therefore a detailed description of the above method seems inappropriate, however, choice of the method should take into account the above considerations. 

Commercial bromelain is currently prepared by centrifugation, ultrafiltration and freeze-drying of cooled pineapple juice, which produces a yellowish powder. Stem bromelain has been generally prepared from the juice of pineapple wastes (mainly stems) by precipitation with organic solvents (e.g., acetone and methanol) or by ultrafiltration. ... 

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