Cellulolytic enzyme lignin

Besides cellulolytic enzyme lignin, the so-called Bjorkman lignin, alternatively referred to as "milled wood lignin" (MWL) is the best preparation known so far, and it has been widely used for structural studies. When wood meal is ground in a ball mill either dry or in the presence of nonswelling solvents, e.g., toluene, the cell structure of the wood is destroyed and a portion of lignin (usually not more than 50%) can be obtained from the suspension by extraction with a dioxane-water mixture. MWL preparations always contain some carbohydrate material. 

Milled Wood Lignin Compared with Cellulolytic Enzyme Lignin... 

Chang H-m, Cowling EB, Brown W, Adler E, Miksche G (1975) Comparative studies on cellulolytic enzyme lignin and milled wood lignin of sweetgum and spruce Holzforschung 29 153-159... 

Fiserova M, Suty L (1980) Comparative studies on milled wood lignins and cellulolytic enzyme lignin of beech wood (Fagus silvatica L ) Cellul Chem Technol 14 243-252 Glasser WG, Barnett CA (1979) The structure of lignins in pulps II A comparative evaluation of isolation methods Holzforschung 33 78-86... 

Bjorkman A, Person B (1957) Studies on finely divided wood. Part 4. Some reactions of the lignin extracted by neutral solvents from Picea abies. Sven Papperstidn 60 285-292 Chang H-M, Cowling EB, Brown W, Adler E, Miksche G (1975) Comparative studies on cellulolytic enzyme lignin and milled wood lignin of sweetgum and spruce. Holzforschung 29 153-159... 

Holtman, K. M., Chang, H. M., and Kadla, J. F. (2004) Solution-state nuclear magnetic resonance study of the similarities between milled wood hgnin and cellulolytic enzyme lignin. J. Agr. Food Chem. 52(4), 720-726. 

The classical wood LCC preparation is considered to be the Bjorkman LCC (or its analogs) isolated from milled wood after preextraction of MWL [24]. Such preparations as MWL and cellulolytic enzyme lignin (CEL) were considered experimental preparations used exclusively to smdy lignin structure. However, more recently, it has been demonstrated that these types of preparations also can be very valuable in the analysis of LCC linkages [21]. 

The reaction was also applied to a sample of specialized cellulolytic enzyme lignin. HSQC analysis of the oxidized lignin revealed that most S units and aU G units were converted to the corresponding ketone. Moreover, preliminary examination of the proposed retro-aldol cleavage of ketone 21 (2 M NaOH, HOOH in 1 1 THF/MeOH at 50°C) afforded an 88% yield of veratric acid and a 42% yield of guaiacol [74]. 

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