Cellular interaction with patterned surfaces

In 2D cell culture on chip, cell adhesion has been extensively studied on patterned surfaces for it is critical to cellular functions. Micropattems have been used to study the cellular interactions with various materials such as metals, polymers, self-assembled monolayers, extracellular matrix proteins, cell adhesion peptides, and other bioactive molecules. The physical and chemical properties of a substrate affect the attachment and growth of cells on it, and many studies have demonstrated that different topographical features of a surface affect cell attachment. Glass, sflicon, and polydimethylsiloxane (PDMS) are widely used as substrate materials of ceU culture microchips. 

In conclusion, the macromolecular properties of polymers and their interactions with cell surfaces result in a specific pharmacokinetic behaviour of polymers. The routes of parenteral administration are far from being equivalent, e.g. the intraperi-toneal application often used cannot substitute the intravenous administration. Molecular parameters of the polymer circulating in the coitral compartment are changed in time not necessarily by a direct biological modification of the polymer but as a consequence of a selective processing of different fractions. The intracellular accumulation in secondary lysosomes is the only proven mode of persistence of a soluble polymer in tissues. Variations in the chemical structure of the polymer may result in a different pattern of polymer distribution in the body as a consequence of a different rate of cellular accumulation. 

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