Cellular adhesion, effect

Cellular Adhesion. The previously discussed effects of IFNy on CD4 cells are complemented by its enhancement of adhesion and morphological alterations on cells that facilitate lymphocyte extravasation to tissue and the expression of CD54 adhesion molecules (ICAM-1) fundamental in... 

Using this method, we constructed a number of dynamic adsorption isotherms for several proteins on different surfaces. Here we present information for two proteins. The adsorption isotherm for bovine serum albumin (BSA) (87% monomer, 13% dimer described by the supplier as 100% monomer) is shown in Figure 6. A plateau concentration for BSA on an amine/ silane surface was 3.5 mg/m2, which is comparable with monolayer adsorption reported previously in a static system (3). [The effect of flow rate, ionic strength, and temperature is reported elsewhere (18).] Perhaps more interesting are the data for plasma fibronectin (produced and purified extensively in our laboratory), because adsorption characteristics of this protein have not been reported elsewhere. Since it is a protein intimately involved in cellular adhesion, its surface behavior is particularly relevant to implant biocompatibility. We found a plateau uptake of approximately 7.0 mg/m2 on both amine and dimethyldimethoxymethylsilane, and based on the assumption of monolayer coverage and a published axial ratio (20), we calculated that the molecule has dimensions of approximately 130 X 80 X 100 A. 

Changes in the composition of the membrane of the oxygenator was also examined as a means of reducing the cellular adhesion to the membrane (1 ). However, the dominant effect was found to be the method by which the membrane of the oxygenator was primed. As with the results described above, the cellular adhesion was in each case significantly reduced by denucleation priming. 

Zeltinger, J., Sherwood, J.K., Graham, D.A., Meller, R., Griffith, L.G., 2001a. Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition. Tissue Eng. 7, 557-572. 

---