CDNA sequencing

Zasloff, M. Magainins, a class of antimicrobial peptides from Xenopus skin Isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc. Nad. Acad. Sci. USA 1987, 84, 5449-5453. 

Amino acid or cDNA sequencing Determination of amino acid sequence. 

Figure 47-10. Schematic diagram of the structure of human L-selectin. The extracellular portion contains an amino terminal domain homologous to C-type lectins and an adjacent epidermal growth factor-like domain. These are followed by a variable number of complement regulatory-like modules (numbered circles) and a transmembrane sequence (blackdiamond). A short cytoplasmic sequence (open rectangle) is at the carboxyl terminal. The structures of P- and E-selectin are similar to that shown except that they contain more complement-regulatory modules.The numbers of amino acids in L-, P-, and E- selectins, as deduced from the cDNA sequences, are 385,789, and 589, respectively. (Reproduced, with permission, from Bevilacqua MP, Nelson RM Selectins. J Clin Invest 1993 91 370.)...

The complete sequence of the pgaX gene has been determined. Seven introns are present in the gene which were all confirmed by cDNA sequencing. The introns are between 50 and 58 nt long, have fungal consensus splice sequences and all but one have in frame stop codons. 

Magert H., Hadrys T., Cieslak A., Groger A., et al. (1995). cDNA sequence, and expression pattern of the putative pheromone carrier aphrodisin. Proc Natl Acad Sci 92, 2091-2095. 

In humans, the structural gene locus is on chromosome 19 (M17), and the gene spans over 40 kilobases (kb) including 18 exons and 17 introns (W2, X2). Neu-roleukin, a protein that acts as both a neurotrophic factor and a lymphokine, has been isolated from mouse salivary glands (G7), and subsequently the primary structure of neuroleukin was found to be identical to that of GPI by comparison of the cDNA sequences (C7, FI). The cDNA sequence encodes 558 amino acid residues. The enzyme consists of two identical subunits with a molecular weight of approximately 63,000 and neuroleukin is active as a monomer. 

The structure and sequence of the catalytic moiety have been determined (06, V6, W6). The enzyme consists of 362 amino acids and 40,638 daltons of the protein predicted by the cDNA sequence. The ADA gene spans 32 kb and consists of 12 exons. The apparent promoter region of the gene lacks the TATA and CAAT sequences often found in eukaryotic promoters and is extremely G/C rich. The location of the ADA gene is on chromosome 20ql2-ql3.11 (Jl). 

N9. Nishi, S Stoffel, M., Xiang, K Shows, T. B Bell, G. I., and Takeda, J Human pancreatic beta-cell glucokinase cDNA sequence and localization of the polymorphic gene to chromosome 7, band p 13. Diabetologia 35,743-747 (1992). 

Sukenaga, Y Ishida, K., Takeda, T., and Takagi, K., cDNA sequence coding for human glutathione peroxidase. Nucleic Acids Res. 15,7178 (1987). 

Simpson, J. C., Wellenreuther, R., Poustka, A., Pepperkok, R., and Wiemann, S. (2000). Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Reports 1, 287-292. 

Next, the difference in the genomic and cDNA sequences of CBP between the F and +Y alleles were determined (Figure 24.5b) (Sakudoh et al. 2005, 2007). The F allele strain had at least two copies of the CBP gene, all of which could be classified into one of two types a F-a sequence... 

Tabunoki, H., Sugiyama, H., Tanaka, Y. et al. 2002. Isolation, characterization, and cDNA sequence of a carotenoid binding protein from the silk gland of Bombyx mori larvae. J. Biol. Chem., 277(35) 32133-32140. 

Urade, Y., et al. (1989). Primary structure of rat brain prostaglandin D synthetase deduced from cDNA sequence. /. Biol. Chem. 264, 1041-5. 

Boesten, W.H.J., Raemakers-Franken, PC., Sonke, T. et al. (2003) Protein and cDNA sequences of -//- -amino acid amide racemases cloned from Ochrobactrum anthropi and Arthrobacter nicotianae, W02003106691. 

Arpagaus, M., Fedon, Y., Cousin, X., Chatonnet, A., Berge, J.-B., Fournier, D. and Toutant, J.-P. (1994) cDNA sequence, gene structure, and in wire expression of ace-1, the gene encoding acetylcholinesterase of class A in the nematode Caenorhabditis elegans. Journal of Biological Chemistry 269, 9957-9965. 

Wu, X., et al. cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family. Biochem. Biophys. Res. Commun. 1998, 246, 589-595. 

Okubo K et al. Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nature Genet 1992 2 173-179. 

The combination of GenBank cDNA sequence comparison and data from the public SNP database reveal the presence of several SNPs in MRP3, many of which involve non-synonymous amino acid changes (Table 9.3). These polymorphisms have not been functionally characterized and, therefore, the clinical pharmacological impact of such MRP3 variants remains unknown. 

---