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Sequencing cDNA from reverse transcripts of RNA

N-terminai aminoacid sequence of human fibroblast interferon MET SER - TYR -A5N LEU [Pg.216]

One likely possibility (Taking into account preferred codon usages) AUG AGC UAC AAC CUG [Pg.216]

5 -end of primer labelled and annealed to crude polyadenyiated mRNA. [Pg.216]

Chain extension with reverse transcriptase. Sequence by Maxam -Gilbert method (chapter 5) [Pg.216]

Sequence corresponding to amino end of interferon precurser peptide [Pg.216]


See other pages where Sequencing cDNA from reverse transcripts of RNA is mentioned: [Pg.214]   


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CDNA sequence

CDNA sequencing

CDNAs

RNA sequences

RNA transcript

RNA transcription

RNA-sequencing

Reverse transcription of RNA

Transcription of RNA

Transcription reverse

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