CDNA sequence analysis

Subsequent to the molecular cloning and cDNA sequence analysis of the delta opioid receptor, antireceptor antibodies were raised against synthetic peptides based on the deduced amino acid sequence of this receptor. To date, there have been a limited number of studies using antibodies directed toward the amino terminus of the cloned murine receptor to assess the distribution of delta opioid receptorlike immunoreactivity in the gastrointestinal tract, and these have been confined so far to the porcine small intestine. They have shown the presence of delta opioid receptor-immuno-reactive neurons and fibers in both the myenteric and submucosal plexuses and as well as in myenteric neurons maintained in primary culture [22, 23]. Receptor-like immunoreactivity in neuronal cell bodies appears to be localized in the cytoplasm and is likely to be trafficked to nerve terminals. These neurons are coimmunoreactive for the acetylcholine-synthesizing... 

Gilmour, S.J., Artus, N.N., and Thomashow, M.F., 1992, cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana. Plant Mol. Biol. 18 13-21. 

FIGURE 2. Organization of the Euglena chloroplast rps4-rpsll Operon determined from chloroplast DNA and cDNA sequence analysis. ... 

Confirmation of the proposed carboxy-terminal sequence was very recently obtained from the nucleotide sequence of a complementary DNA (cDNA) segment coding for the carboxy-terminal portion of RBP (Costanzo et al., 1983). A human liver cDNA library was constructed and a large number of individual clones were screened directly by cDNA sequence analysis. One of the 236 sequences screened coded for the region of human RBP from amino acid residue 159 to 182. 

Blackburn, D.E., Hobbs, A.A., and Rosen, J.M. (1982) Rat beta casein cDNA sequence analysis and evolutionary comparisons. Nudeic Acids Res.,... 

Fagan, T. F., et al. (1993). Cloning, expression and sequence analysis of cDNA for the Ca2+-binding photoprotein, mitrocomin. FEBS Lett. 333 301-305. 

Cloning of a Fruit BSubunit cDNA and Analysis its Primary Sequence... 

Kuhn N., Woodworth-Gutai M., Gross K. and Held W. (1984). Subfamilies of MUP multi-gene family sequence analysis of cDNA clones and diffferential regulation in... 

Leibovitch, S. A., Lenormand, J. L., Leibovitch, M. P., Guiller, M., Mallard, L., and Harel, J. (1990). Rat myogenic c-mos cDNA cloning sequence analysis and regulation during muscle development. Oncogene 5 1149-1157. 

Okubo K et al. Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nature Genet 1992 2 173-179. 

Sequence analysis of cDNA clones shows that the 540-bp consensus repeats on DNA segment B, H, and O1 are contained within an open... 

The amino acid sequence has been partly determined on the purified protein and was fully deduced from the sequenced gene 7,35. According to this analysis the mature CBH II protein sequence is 447 amino acids long, the numbers of amino acid residues deduced from the cDNA sequence per CBH II molecule being as follows 56 Ala, 13 Arg, 30 Asn, 21 Asp, 12 21 Gin, 8 Glu, 39 Gly, 4 His, 15 He, 32 Leu, 10 Lys,... 

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