Vectors cationic lipid

Tan Y, et al. The inhibitory role of CpG immunostimulatory motifs in cationic lipid vector-mediated transgene expression in vivo. Hum Gene Ther 1999 10 2153. 

Due to the complexity of the transfection pathway, no general schemes have evolved for correlating cationic lipid chemistry with transfection activity. A useful approach for designing and optimizing the cationic lipid vectors, as well as for... 

Li S, Tseng WC, Stolz DB, Wu SP, Watkins SC, Huang L (1999) Dynamic changes in the characteristics of cationic lipidic vectors after exposure to mouse serum implications for intravenous lipofection. Gene Ther 6 585-594... 

Bouxsein NF, McAllister CS, Ewert KK, Samuel CE, Safinya CR (2007) Structure and gene silencing activities of monovalent and pentavalent cationic lipid vectors complexed with siRNA. Biochemistry 46 4785 -792... 

Hirko, A., F. Tang, and J.A. Hughes. 2003. Cationic lipid vectors for plasmid DNA delivery. Curr. Med. Chem. 10 1185-1193. 

I. S. Zuhorn, J. B. F. N. Engberts, D. Hoekstra, Gene deliveiy by cationic lipid vectors overcoming cellular barriers, Eur. Biophys. J. Biophys. Lett., QIXTI, 36, 349—362. 

The use of responsive vectors highlights the multifaceted and complex nature of these cationic lipid vectors and the supramolecular structures they form with DNA. By varying composition, chain lengths, linkers, and various properties, the lipoplex phase can be tailored to improve gene delivery. The research featured in this chapter has shown how the use of responsive nonviral vectors can enhance endosomal escape of the lipoplex and release of DNA from the lipoplex system, subsequently affecting gene transfer. 

Li S, Wu SP, Whitmore M, Loefifert EJ, Wang L, Watkins SC, et al. Effect of immime response on gene transfer to the lung via systemic administration of cationic lipidic vectors. Am J Physiol 1999 276 L796-L804. 

Filion, M.C., and Philips, N.C., Toxicity and immunomodulatory activity of liposomal vectors formulated with cationic lipids toward immune effector cells, Biochimica et Biophysica Acta, 1997, 1329, 345-356. 

It should be emphasized at this point that the use of physicochemical methods is so far the only way to demonstrate the import of transgene DNA into the mitochondrial matrix in living mammalian cells. The unavailability of a mitochondria-specific reporter plasmid designed for mitochondrial expression severely hampers current efforts toward the development of effective mitochondrial expression vectors. Although any new nonviral transfection system (i.e., cationic lipids, polymers, and others) aimed at the nuclear-cytosolic expression of proteins can be systematically tested and subsequently improved by utilizing anyone of many commercially available reporter gene systems, such a methodical approach to develop mitochondrial transfection systems is currently impossible. 

Vigneron, J.P., Oudrhiri, N., Fauquet, M., Vergely, L., Bradley, J.C., Basseville, M. et al. (1996) Guanidinium-cholesterol cationic lipids Efficient vectors for the transfection of eukaryotic cells. Proc. Natl. Acad. Sci. USA, 93, 9682-9686. 

Compared to viral vectors, the potential advantages of synthetic carriers (also called non-viral vectors) are apparent. Being synthetic, they could be made safe, non-immunogenic, easy to prepare and cost-effective. DNA delivered by these carriers may not be able to replicate or recombine into infectious forms. Among many reported non-viral carriers, including cationic polymers (Behr et al., 1989 Kukowska-Latallo et al., 1996 Wu and Wu, 1988) and cationic lipids (Feigner, 1990 Lee and Huang, 1997), the most frequently used form is cationic liposomes. 

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