Carcinoma embryonic antigen

Badcock, G., Pigott, C., Goepel, J., Andrews, P. W. (1999). The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res., 59, 4715-4719. 

Conry RM, Curiel DT, Strong TV, Moore SE, AUen KO, Barlow DL, Shaw DR, LoBuglio AF. Safety and immunogenicity of a DNA vaccine encoding carcino-embryonic antigen and hepatitis B surface antigen in colorectal carcinoma patients. Clin Cancer Res 2002 8 2782-2787. 

In Table 6-2, peak intensities of the three types of tissues are compared. It is seen that the intensities at 1,039 and 1,076 cm-1 increase in going from normal to cirrhotic to cancerous tissues. The same trend is seen in the intensity ratio of the 1,182/1,156-cm-1 bands. Since RBC contributions to these two bands are similar, this ratio can also be used for cancer diagnosis. The increased intensity of the 1,182-cm-1 band may indicate an increase in production of a-fetoprotein, which is a specific antigen for hepatoma and embryonal carcinoma. The band at 1,039 cm-1 may be due to the phenylalanine residue. Finally, the band at 1,076 cm-1 is due to the symmetric PO2 vibration of the DNA backbone (Section 4.1.2), and its increase in Raman intensity indicates an increase in the DNA concentration in cancer tissues. 

Andrews, P. W., Banting, G., Damjanov, I., Amaud, D., Avner, P. (1984). Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma, 3, 347-361. 

PLAP, placental alkaline phosphatase CDl 1 7 (c-kit), membrane staining CK, cytokeratin, rare dysgerminomas showweak staining, with AEl /AE3 embryonal carcinoma shows membrane staining and yolk sac tumor cytoplasmic staining LCA, leukocyte common antigen MPO, myeloperoxidase. 

Linzer DI, Levine AJ (1979) Characterization of a 54 K Dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells. Cell 17 43-52... 

Cytokeratin 8 (CK 8) is an intermediate filament type II and one of the first cytokeratins expressed in the embryonal tissue and persists throughout the adult tissue. CK 8 is encoded on chromosome 12 and expressed in the vast majority of adenocarcinomas including lung, breast, gastrointestinal adenocarcinoma in addition to transitional, neuroendocrine and hepatocellular carcinoma, but is not detectable in squamous epithelium. CK 8 is also known as tissue polypeptide antigen (TPA) detectable in serum of cancer patients. 

The role of cell-surface glycoproteins in malignant transformation has been discussed. An outline of the teratoma system in relation to cell-surface antigens of mouse embryonal carcinoma cells has been published. ... 

---