Carbol fuchsin

The surface structure of gram-negative bacteria (these are not stained by Gram s method and must be stained red with carbol fuchsin) is more diversified. It consists of an outer membrane whose main building unit is a lipopolysaccharide together with phospholipids and proteins. The actual cell... 

Flood the slide with Kinyon carbol-fuchsin, and stain the smear for 5 min. 

Stains.—One of the most useful bacteriologic stains is ZiehVs Carbol Fuchsin, prepared as follows ... 

I. Flood the cover glass with Ziehl s carbol fuchsin and boil over the flame for thirty seconds. 

Add glutaraldehyde to plate three for 2 h, remove agarose overlay, and add carbol fuchsin to each well for 30 min. Wash off stain and count plaques to determine the titer of each virus. 

Cryptosporidium species malachite green, safranine, methylene blue, carbol-fuchsin, auramine-rhodamine, IFA... 

Not all bacteria can be stained by the Gram procedure, however, and these often require special staining processes for visualization. Among the more prominent of these for our purposes are the mycobacteria (the causative agents of tuberculosis, for example). These very waxy cells are called acid-fast and are stained by carbol fuchsin instead. 

Ziehl-Neelsen s carbol fuchsin may be prepared by dissolving 10 g of basic fuchsin in 100 ml of absolute ethanol and then adding this preparation to one litre of 5% phenol in water. 

Alieva, E. I. Filin, V. A. Shcherbakov, 1.T. Gracheva, N. M. Leont eva, N. I. Partin, O. S. Novikova, A. V. Method for diagnosis of cryptosporidiosis involving histological exami-nation of intestinal mucosa bioptates fixed with methanol, stained with carbolic fuchsine, and dechlorinated with sulfuric acid. Russ. RU 2229128, 2004 Chem. Abstr. 2004,141,172330. 

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