Carbohydrate processing enzymes

T. M. Wrodnigg, A. J. Steiner, and B. J. Uberbacher, Natural and synthetic iminosugars as carbohydrate processing enzyme inhibitors for cancer therapy, Anticancer Agents Med. Chem., 8 (2008) 77-85. 

Lessons from these results are that carbohydrate processing enzymes can be optimized by site-directed and random mutagenesis in a more rational way for the production of tailored oligo- and polysaccharides under various aspects, also including up- and downstream processing for industrial purposes. 

In conjunction with our overview of glycomimetics that block protein-carbohydrate interactions, we also discuss strategies to develop inhibitors of carbohydrate-processing enzymes this section focuses on the enzymes involved in bacterial cell wall biosynthesis because it is an area in which many new advances have been made. Enzymes that utilize sugars and synthesize glycoconjugates unique to pathogens have been identified, and cell-permeable inhibitors can be used to explore their biological roles or validate a potential... 

Overview and Future Development Inhibitors of Carbohydrate-processing Enzymes... 

The preparation of sugar amidines, amidrazones and amidoximes as inhibitors of carbohydrate-processing enzymes has been reviewed. Treatment of 2-deoxy-o-arabino-hcxose oxime with chloramine-T gave 2-deoxy-D-arafeino-hexono-l,4-oximinolactone, but the intermediate nitrones formed from this and 2-deoxy-o-... 

Amino- and Imino-alditols - Reviews on the syntheses of azasugars (imi-noalditols) from non-carbohydrate sources on the syntheses of azasugars, sugar amidines and related structures as inhibitors of carbohydrate-processing enzymes on the syntheses of glycosidase inhibitors with reference to nag-statin and on the use of aldolases in the chemo-enzymatic syntheses of iminoalditols have appeared. 

Let us consider the practical use of the two types of carbohydrate processing enzymes in more detail. 

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