Canola Antioxidant Canolol

For example, the main phenol with antioxidative and antimutagenic activities that occurs in crude canola oil is 2,6-dimethoxy-4-vinylphenol known as canolol, which is produced from syringic acids. The major products of caffeic acid pyrolysis at 225 °C are pyrocatechol, 4-vinylpyrocatechol and 4-ethylpyrocatechol (see Section 8.3.4). A more complex mixture of products arises by pyrolysis of lignin, in the smoke condensates used in the meat industry, more than 150 different phenols, and dozens of aromatic alcohols, phenolic acids and hydroxylated heterocyclic compounds have been identified. 

Kuwahara H., Kanazawa A., Wakamatu D., Morimura S., Kida K., Akaike T., Maeda H. Antioxidative and antimutagenic activities of 4-vinyl-2,6-dimethoxyphenol (canolol) isolated from canola oH. Journal of Agricultural and Food Chemistry, 52 4380-4387 (2004). 

More recently, another derivative of sinapic acid has been identified because of its highly potent antioxidant activity and the antimutagenic and anticarcino-genic properties (Kuwahara et al., 2004 Vuorela et al., 2005 Cao et al., 2008). This derivative, 2,6-dimethoxy-4-vinylphenol (vinylsyringol) or canolol, was first identified in two independent studies by Koski et al. (2003) and Wakamatsu et al. (2005) in crude canola oil. Kuwahara et al. (2004) described the antimutagenic potency of this compound as higher than a-tocopherol, P-carotene, vitamin C... 

In the literature, canolol is described as an effective antioxidant (Koski et al., 2003 Kuwahara et al., 2004 Vuorela et al., 2005 Wakamatsu et al., 2005). Figure 17.8 shows increased oxidative stability of canola oil accompanied increasing amounts of canolol. Using the Rancimat test at 120°C canolol contents below 200 itg/g in canola oil from roasted seeds resulted in slight decrease of the oxidative stability. In contrast, amounts of canolol higher than 200 g/g improved oxidative stability of the oil dramatically. Wijesundera et al. (2008) also showed an improvement in... 

To assess the antioxidant activity of canolol, it is necessary to isolate canolol from canola oil by extraction from roasted seeds. For the extraction, about 50 g of oil was diluted with 200 mL mixture composed of ethylacetate/cyclohexane (1 1). From this solution, 7 mL was used for purification by gel permeation chromatography (GPC). The purity of the extract was established by HPLC with recalculation of the nsed amount of extract. From 1.5 g oil, 3 mg purified extract was obtained. 

Given the broad spectrum and the potent which is antioxidative in nature, besides being a good antioxidant in lipids, canolol can effectively be used as a dietary supplement in combating oxidative stress in living organisms. Canolol, as a constituent of canola/rapeseed oil, has already been residual mostly in crude oils. In this context, it can be presumed well that enriching oils with canolol will benefit the value of foods. Nevertheless, to further substantiate its claim, in disease and nutrition, human clinical trials would be necessary. If human trials are in line to the in vitro and in vivo studies, canolol can be positioned as a new powerful nutraceutical and hit the market in future. 

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