Calcium nuclear magnetic resonance

The binding of calcium ion to calmodulin, a major biochemical regulator of ion pumps and receptors, occurs on a time scale about a thousand times shorter than that observed for RNA conformational change. This Ca2+-calmodulin binding, which can be followed successfully by nuclear magnetic resonance (NMR), occurs in about ten milliseconds. 

K. B. Seamon, Calcium- and magnesium-dependent conformational states of calmodulin as determined by nuclear magnetic resonance, Biochemistry 19, 207-215 (1980). 

J. P. Yesinowski, Nuclear magnetic resonance spectroscopy of calcium phosphates, in Z. Amjad (Ed.), Calcium Phosphates in Biological and Industrial Systems, Kluwer Academic Publishers, Norwell, MA, 1998, pp. 103-143. 

Y. Pan, P- F rotational-echo double resonance nuclear magnetic resonance experiment on fluoridated hydroxyapatite. Solid State Nucl. Magn. Reson. 5 (1995) 263-268. L. Wu, W. Forsling, P.W. Schindler, Surface complexation of calcium mineral in aqueous solution, surface protonation at fluorapatite surface, J. Colloid Interface Sci. 147 (1991) 178-185. 

K. J. D. MacKenzie and R. H. Meinhold, Thermal decomposition of dolomite (calcium magnesium carbonate) studied by Mg solid-state nuclear magnetic resonance. Ther-mochim. Acta, 1993, 230,331-337. 

Drohat AC, Baldisseri DM, Rustandi RR, Weber DJ. 1998. Solution structure of calcium-bound rat S100B(betabeta) as determined by nuclear magnetic resonance spectroscopy. Biochemistry 37(9) 2729-2740. 

Aue WP, Roufosse AH, Gitmcher MJ, Griffin RG (1984) Solid-state phosphorus-31 nuclear magnetic resonance studies of synthetic solid phases of calcium phosphate potential models of hone mineral. Biochemistry 23 6110-6114... 

The interaction between troponin C and troponin T was first observed as the Ca -dependent turbidity change of the suspension of troponin C and troponin T (Ebashi et al, 1973). But the range of Ca concentration which caused the turbidity change was higher than that required for contractile regulation. A calcium-sensitive interaction with troponin T was also reported in a study of nuclear magnetic resonance (Ohnishi et al., 1975). 

In this chapter common methods to evaluate chemical properties and phase composition of bioceramic coatings will be briefly described that are available in many laboratories including X-ray diffraction (XRD), vibrational spectroscopy techniques such as infrared (FTIR) and Raman spectroscopy and nuclear magnetic resonance spectroscopy (NMR). These methods provide a host of information on bulk phase composition, degree of crystallinity and crystallite size. Some special techniques including cathodoluminescence serve to reveal intrinsic coating properties that cannot be assessed by conventional analytical techniques, for example to distinguish between amorphous calcium phosphate (ACP) and crystalline calcium phosphates. 

The nuclear magnetic resonance technique is very useful for the study of a metal-loprotein, namely to obtain information about the changes of conformation, redox states, assignment of axial ligands in heme proteins and detection of different spin states. H-NMR was used to study the oxidized and the half-reduced states of P. denitrificans CCP in the presence and absence of calcium. The results have confirmed the hypothesis based on interpretation of the UV/visible spectra and have also contributed to add new data to the mechanism of activation of the protein. Figure 6-5 shows the 300 MHz H-NMR of the oxidized and half-reduced states... 

Grutzeck, M., Benesi, A., and Fanning, B., Silicon-29 magic angle spinning nuclear magnetic resonance study of calcium silicate hydrates, J. Am. Ceram. Soc., 72 (4), 665 (1989). 

Equation (53) describes Debye relaxation. Magnesium and calcium-doped lithium fluorides have a characteristic Debye relaxation diagram from vhich the dopant concentration and the relaxation time can be deduced. Many others crystals containing mobile lattice defects have similar Debye s relaxation processes. Major understanding of the structure of color centers results from dielectric relaxation spectra. Nuclear magnetic resonance, optical and Raman spectroscopy can be used efficiently in conjunction vith dielectric spectroscopy. 

S. and Andreu, L. (2000) Calcium- and iron-related phosphorus in calcareous and calcareous marsh soils sequential chemical fractionation and P nuclear magnetic resonance study. Communications in Soil Science and Plant Analysis 31, 2483-2499. 

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