Caffeine from cola syrup

Many of the chemicals used in this course will be unfamiliar to you. Their properties can be looked up in reference books, a very useful one being the Aldrich Catalog Handbook of Fine Chemicals. It is interesting to note that 1,4-dichlorobenzene is listed as a toxic irritant and naphthalene is listed as an irritant. Both are used as moth balls. Camphor, used in vaporizers, is classified as a flammable solid irritant. Salicylic acid, which we will use to synthesize aspirin (Chapter 26) is listed as moisture-sensitive toxic. Aspirin (acetylsalicyclic acid) is classified as an irritant. Caffeine, which we will isolate from tea or cola syrup (Chapter 8), is classified as toxic. Substances not so familiar to you—1-naphthol and benzoic acid—are classified respectively as toxic irritants and irritant. To put things in some perspective, nicotine is classified as highly toxic. ... 

Extraction Isolation of Caffeine from Tea and Cola Syrup... 

Automatic soft drink dispensing machines mix a syrup with carbonated water. In the following experiment caffeine is extracted from concentrated cola syrup. 

HPLC on an octadecyl column can be applied to the analysis of caffeine from tea or cola syrup (Chapter 8—use 20% methanol, 0.8% acetic acid, and 79.2% water as eluant), the acetylation of cholesterol (Chapter 10) pulegone and citronellal from citronellol (Chapter 25), cholesterol from gallstones (Chapter 22), the isolation of eugenol from cloves (Chapter 28— use 10% methanol, 5.4% acetic acid, and 84.6% water as eluant), isolation of lycopene and /3-carotene (Chapter 9), and the product obtained from enzymatic reduction of ethyl acetoacetate (Chapter 59). 

---