Cabbage phospholipase

L.R. Juneja, N. Hibi, N. Inagaki, T. Yamane, and S. Shimizu Comparative study on conversion of phosphatidylcholine to phosphatidylglycerol by cabbage phospholipase D in micelle and emulsion system. Enzyme and Microbial Technology 9 (1987) 350-354. 

Phospholipase D Cabbage Triton X-lOO/phosphotidyl choline/diethyl ether Enzyme characteristics [87]... 

An acetone powder of Savoy cabbage leaves (prepared by heat coagulation and acetone precipitation and available from commercial sources) is suspended in diethyl ether and then filtered. The residue is dispersed in sodium acetate and calcium chloride at pH 5.0, and the insoluble matter is separated by centrifugation at 13,000 g for 10 min at 4°C. The supernatant contains all the phospholipase D activity and in our laboratory s experience can be used successfully in any experiment requiring this enzymatic activity. It can be maintained at -20°C for 1-2 weeks. 

In another detailed study on the influence of the substituent at the C-l position in sn-3 choline phosphoglycerides, Waku and Nakazawa (1972) investigated the catalytic behavior of the phospholipase D of cabbage and carrot. Their findings showed that either source exhibited high activity toward a diacylphosphoglyceride, with a much lower rate of hydrolysis toward an alkylacyl- and alkenylacylphosphoglyceride. 

Phospholipase D. This enzyme will attack phosphatidylserine with the liberation of serine and formation of phosphatidic acid. The methodology is exactly the same as the one outlined in Chapter 4. The source of enzyme can be Streptomyces chromofuscus or cabbage, and products of its action are recovered in a chloroform-soluble and a water-soluble fraction. All of the lipid P should be in the chloroform-soluble fraction, and all of the serine should be in the water-soluble fraction. The phosphatidic acid can be identified by its thin-layer chromatographic behavior and its fast atom bombardment-mass spectrometric pattern. Serine can be identified by the procedures outlined earlier. 

Allgyer, T. J. and Wells, M. A. (1979) Phospholipase D from Savoy cabbage purification and preliminary kinetic characterization, Biochemistry 18, 5348-5353. 

C. Vitro, I. Svensson, and P. Adlercreutz Hydrolytic and transphosphatidylation activities of phospholipase D from savoy cabbage towards ly sophosphatidylcholine. Chemistry and Physics of Lipids 106 (2000) 41-51. 

Davidson, F. M., and C. Long The structure of the naturally occurring phosphoglycerides. 4. Action of cabbage-leaf phospholipase D on ovolecithin and related substances. Biochem. J. 69, 458—66 (1958). 

Plants are the main source for phospholipase D although an enzyme catabolizing ether lipids has been found in brain and other tissues. The plant enzymes have been purified from several tissues such as cabbage or carrots. In addition to catalysing hydrolysis of phospholipids the enzyme will cause phosphatidate exchange. This allows the formation of new phospholipids in the presence of an appropriate alcohol. For example ... 

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