Buffered paper

The conditions for a satisfactory run include a series of physical factors in connection with the electrophoretic chamber, the paper, the buffer, and the electrical field. While each of these has its separate effect, the interaction among them must be considered, for it is the electrical current passing through a buffered paper strip that causes electrophoretic migration. 

Electrophoresis on borate-buffered paper is potentially useful for the detection of cyclitols. So far, however, the principal application of this technique in the cyclitol field has been to studies on configuration and on the nature of cyclitol-borate complexes (see p. 153). 

However, much can be done to minimize damage caused on the shelves or by library users if the material is stored in acid-free folders. They are easily fabricated and many types suitable for archive use are available commercially. It is essential that clearly acid papers be stored separately from nonacid ones in order to prevent migration of the acid. Some conservators place a sheet of buffered paper in the folder to absorb free acid from the print. 

Application of the electric field and the resultant resistance to current flow in the buffered paper generates heat. This is the greatest source of difficulty with paper electrophoresis. Heat dries the paper, which in turn leads to more resistance to current flow, which causes greater resistance, and so forth. 

It is not difficult to guess why the time-ratios in Figure 3 increase as degradation proceeds. The cold extraction pH of the acidic paper was found to decrease during the degradation process, reaching pH 3.8 by the end of the experiment. The pH of the buffered paper remained constant. Therefore, it seems probable that the increased value of the time-ratio as degradation occurred is a manifestation of an autocatalytic process. Similar results were obtained in the comparison of samples of a buffered and a not-buffered rag paper. Thus, the autocatalysis process... 

For the storing of textiles on new wooden shelving or drawers, a coating of a polyurethane varnish may be applied to seal the wood. Sufficient time, up to a month, should be allowed for the coating to dry. The shelves then can be lined with acid-free, buffered papers purchased from archival paper supply firms. These papers liners can be cut to fit the contour of any object and so need not to be noticeable in a display. 

In principle two types of electrode buffers are in use dry, non-aqueous solvents, or mixtures of organic solvents with aqueous buffers. Paper serves as supporting medium for electrophoresis. 

Levy, A. L., and Chung, D. (1953). Two-dimensional chromatography of amino acids on buffered papers. Anal. Chem. 25 396-399. 

B. Ethyl acetate satd. with aq. sodium acetate buffer (0.1 A/ to Na) pH 5.2 vs. paper pretreated with the buffer. Paper is soaked in buffer, blotted, air dried and used immediately. Solvent reaches bottom of paper in 3h, but develop 18 h... 

C. n-Butanol satd. with water vs. pH 4 buffered paper... 

Whatman No. 1. Buffer paper with Mcllvaine s buffer, pH 3.5, blot firmly, spot antibiotics and develop in solvent while still damp Solvent... 

Prouvost, L. and Quintard, M. 1987. Stability Criteria for the Design of Graded Polymer Buffers. Paper SPE 15976 available from SPE, Richardson, Texas. 

Christy CD, Collings K, Drummond P et al (2004) A mobile sensor platform for measurement of soil pH and buffering. Paper 041042. American Society of Agricultural and Biological Engineers, St. Joseph, MI... 

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