Buckwheat protein

Tanaka K, Matsumoto K, Akasawa A, et al. (2002). Pepsin-resistant 16-kD buckwheat protein is associated with immediate hypersensitivity reaction in patients with buckwheat allergy. Int. Arch. Allergy Immunol, 129 49-56. 

FASPEK KIT for buckwheat Soluble peanut protein mixture... 

Buckwheat Allergen checker [Buckwheat] Oriental Yeast Co., Ltd. Gene encoding soba allergenic protein 127... 

Cereal flour, buckwheat flour, soy flour, seafood allergens, pork, sesame seeds, sunflower seeds, lupin, spinach, sarsaparilla root dust, cocoa, coffee dusts, green tea, egg protein, lactalbumin, milk powder, casein, honey, a-amylase, glucoamylase, pectinase, gluconase, pepsin, pectin, spices, carmine, flavorings... 

Podolska, G., Konopka, I., and Dziuba, J. 2007. Response of grain yield, yield components and allergic protein content of buckwheat to drought stress. Advances in buckwheat research. Proceedings of the 10th International Symposium on Buckwheat. Northwest A and F University. China, August 14-18, 2007, 323-328. 

Thiol groups in proteins also react with water soluble carbodiimides. For example, papain is modified at cysteine 25. Also, free thiol groups in buckwheat a-glucosidase are protected by reacting them with a carbodiimide. Tyrosine residues are also modified with carbodiimides, and the inactivation of yeast hexokinase is reversed by hydroxylamine. ... 

Buckwheat Gene encoding soba allergenic protein... 

The FASTKIT ELISA Ver. II (buckwheat) used polyclonal antibodies against whole buckwheat soluble protein. The limit of detection (LOD) for FASTKIT ELISA Ver. II (buckwheat) is 0.39 ng/mL, equivalent to 0.15-mg/kg samples, and the limit of quantification (LOQ) is 1.56 ng/mL, equivalent to 0.62-mg/kg samples. The FASPEK KIT for buckwheat used polyclonal antibodies against the purified main protein complex of buckwheat. The LOQ is 0.78 ng/mL, equivalent to 0.31-mg/kg samples. 

In addition, we conducted the interlaboratory validation study for these kits. The results are shown in Table 15.6. The data suggest that both ELISA kits are precise and reliable tools for the quantification of buckwheat soluble protein in processed foods. 

TABLE 15.6 Recoveries, Repeatabilities, and Reproducibilities for Buckwheat-Soluble Proteins... 

Wang Z, Zhang Z, Wieslander G, et al. (2000). Purification and some properties of the protein with 24kDa in Tartary buckwheat. Fagopyrum, 17 41-44. 

Yoshioka H, Ohmoto T, Urisn A, Mine Y, Adachi T (2004). Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat, i. Plant Physiol, 161 761-161. 

Koyono S, Takagi K, Teshima R et al. (2006). Molecular cloning of cDNA, recombinant protein expression and characterization of a buckwheat 16kDa major allergen. Int. Arch. Allergy Immunol 140 73-81. 

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