Buckwheat-soluble proteins

The FASTKIT ELISA Ver. II (buckwheat) used polyclonal antibodies against whole buckwheat soluble protein. The limit of detection (LOD) for FASTKIT ELISA Ver. II (buckwheat) is 0.39 ng/mL, equivalent to 0.15-mg/kg samples, and the limit of quantification (LOQ) is 1.56 ng/mL, equivalent to 0.62-mg/kg samples. The FASPEK KIT for buckwheat used polyclonal antibodies against the purified main protein complex of buckwheat. The LOQ is 0.78 ng/mL, equivalent to 0.31-mg/kg samples. 

In addition, we conducted the interlaboratory validation study for these kits. The results are shown in Table 15.6. The data suggest that both ELISA kits are precise and reliable tools for the quantification of buckwheat soluble protein in processed foods. 

TABLE 15.6 Recoveries, Repeatabilities, and Reproducibilities for Buckwheat-Soluble Proteins... 

FASPEK KIT for buckwheat Soluble peanut protein mixture... 

Thiol groups in proteins also react with water soluble carbodiimides. For example, papain is modified at cysteine 25. Also, free thiol groups in buckwheat a-glucosidase are protected by reacting them with a carbodiimide. Tyrosine residues are also modified with carbodiimides, and the inactivation of yeast hexokinase is reversed by hydroxylamine. ... 

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