BSA nanoparticle

Fig. 3 Stability of PLL-coated BSA nanoparticles based on release profile of FITC-BSA from the nanoparticles in phosphate buffer (pH 7.4). PLL of molecular mass 0.9 kDa (a), 4.2 kDa (b), 13.8 kDa (c), and 24 kDa (d) at concentrations of 0.1 mg/mL (filled circles), 0.3 mg/mL (open circles), and 1.0 mg/mL (filled inverted triangles) were used for coating onto BSA nanoparticles [107]...

Preparation of Size-Controlled BSA Nanoparticles by Intermittent Addition of... 

Keywords— BSA nanoparticle, coacervation method, dynamic light scattering, drug deUvery. 

BSA nanoparticles were prepared using a desolvation method [9][12][18][19][20], In previous study, we set up a condition to prepare 200 nm sized BSA nanoparticles. BSA powder was added to distilled water. Ethanol was added continuously or intemiittently into 1% BSA solution at pH 7 under stirring at 700 rpm at room temperature until the solution became just turbid. Ethanol was added continuous in the solution with rate addition about 1.0 to 2.0 ml per min for continuous method and the rest time for each mL of ethanol addition was 5 min for intermittent method. 

Size, number, and volume distribution of BSA nanoparticles were measured by commercial particle size analyzer (DelsaNano, Beckman Coulter, Inc., Fullerton, CA, USA). The particle size was measured at 25°C with a scattering angle of 165°. 

The similar problems were occurred in our research for BSA nanoparticle fabrication with continuous ethanol addition. Herein, we suggest that the intermittent addition of desolvating agent can improve reproducibility of BSA nanoparticle preparation. 

Jahartshahi M, NajaljKmr G, Rahimnejad M. (2008) Applying the Taguchi method for optirrtized fabrication of bovine serum albumin (BSA) nanoparticles as drug delivery vehicles. Afr. J. Biotechnol, 7(4), 362-367... 

Fig. 10 Multifunctional solid biodegradable PLGA nanoparticles attached to several moieties such as T-cell antibodies, magnetic resonance contrast agent (biotin-BSA-Gd-DTPA) and encapsulated immunosuppressive drug (doxorubicin). (Adapted from [48])...

The fluorescent labels reported for investigation of intracellular uptake and distribution by CLSM comprise Nile red [13], Texas Red, and 6-coumarin [14]. Not only for fluorescence microscopy but also for transmission electron microscopy (TEM), the loading of markers proved to be useful. Osmium tetroxid as an electron dense marker and bovine serum albumin (BSA) as a model protein were entrapped in PLGA-nanoparticles to elucidate their uptake and intracellular distribution in human vascular smooth muscle cells [15]. 

Agin et al. [83] Hypoxanthine Sardines Chicken Xanthine oxidase/cross-linked with glutaraldehyde and BSA Carbon paste electrode modified with electrodeposited gold nanoparticles/O V vs. Ag/ AgCl ... 

One phase contains the polysaccharide chitosan (CS) and a diblock copolymer of ethylene oxide and the polyanion sodium tripolyphosphate (TPP). It was stated that the size (200-1000 nm) and zeta potential (between + 20 mV and + 60 mV) of nanoparticles can be conventionally modulated by varying the ratio of CS/PEO to PPO. Furthermore, using BSA as a model protein, it was shown that these new nanoparticles have a high protein loading capacity (entrapment efficiency up to 80 % of the protein) and provide a continuous release of the entrapped protein for up to 1 week [56]. 

Fig. 5. Effect of initial BSA concentration and pH of CT solution on entrapment efficiency (mean SD, n = 3) for CT/TPP nanoparticles (reproduced from Calvo et al. 1997, with permission of Plenum Press [15])...

FIGURE 3 TEM photographs of BSA-loaded BCDen47PLA nanoparticles prepared with different techniques and their magnified images. (Reprinted from H. Gao, Y. W. Yang, Y. G. Fan, and J. B., Ma, Journal of Controlled Release, 112, 301-311, 2006. Copyright 2006, with permission from Elsevier.)... 

Fig. 10 Scanning electron micrograph of BSA-loaded PLGA nanoparticles. (With permission from Ref...

The release characteristics of nanocontainers with the polyelectrolyte shell (Fig. Ic) were studied in aqueous neutral solutions under laser irradiation. It is seen from Fig. Id that under the dark conditions the release of eneapsulated BSA is almost completely suppressed. The release of BSA under IR laser irradiation was observed for Si02- and Ti02-based containers modified with Ag nanoparticles, while only Ti02-based containers (both bare and silver-modified ones) exhibit switching into the open state under UV irradiation. It is seen from Fig. Id that the UV-induced release appears to be especially effective in the case... 

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