Brevundimonas diminuta

Several bacterial strains utilizing isoquinoline have been isolated, which metabolized isoquinoline via l(2H)isoquinolinone, such as Brevundimonas diminuta strain 7. 

In 1987, FDA published its guideline on validation of aseptic processing [43] specifying requirements for challenging filters with 107 cells of Pseudomonas (now Brevundimonas) diminuta per cm2 of fdter surface and for validating aseptic processes using sterile media fills. 

Hydrophilic and hydrophobic sterilization grade filters are used throughout the BFS process for the sterilization of product and air, respectively. Filters should be purchased from an approved supplier and should be certified as meeting the regulatory requirements for sterilizing grade filters. By definition this means that the filter will have full bacterial retention when subjected to an aqueous challenge of Brevundimonas diminuta (ATCC 19146) at a minimum concentration of 1 X 10 cfu/cm of filter surface area. 

The FDA defines sterilizing filters as those that have pore-size ratings of 0.22 pm or smaller and relates this to a microbiological particle passage test using Pseudomonas (Brevundimonas) diminuta ... 

Aminobacter aminovorans Brevundimonas diminuta Burkolderia cepacia... 

Filter tests must be performed by the manufacturer before release to the market and by the individual user also, to ensure that the membrane filter complies with the specifications, is undamaged, and is eventually placed correctly in the filter case by the user. As described before in this chapter, such a test should in fact be performed with Brevundimonas diminuta. However, in daily practice this is not possible for the user in the pharmacy, and thus test methods have been derived, which are based on the physical properties of the membrane filter. Such test methods are called filter integrity tests. 

Brevundimonas diminuta PTE is a 72-kDa dimeric bimetallic enzyme with Zn + involved in the catalytic process (Carletti et al., 2009). Substituhon of the native Zn + ions in the active site with Mn, Co, Ni, or Cd ions results in the almost full retention of cafalyhc activity. After tire first determination of the three-dimensional structure of P. diminuta PTE (Benning et al., 1994), a series of crystal structures and kinetic and spectroscopic experiments were described. Nevertheless, the enzyme mechanism of bacterial PTEs is still debated and the functional roles of divalent metal cations and amino acids in the active center are not yet fully understood (Aubert et al., 2004 Jackson et al., 2006,2008 Chen et al., 2007 Samples et al., 2007 Wong and Gao, 2007 Bigley and Raushel, 2013). 

Synthesis of (S)- and (/ )-a-amino acids using nitrile hydratase (NHase) from Rhodococcus opacus, a-amino-e-caprolactam racemase (ACL) from Achromobacter obae, and different amidases (amidase 1, o-amino-peptidase from Ochrobactrum anthropi amidase 2, i-amino acid amidase from Brevundimonas diminuta) [111, 112]. / =CH3, CH CH, CH3CH(CH3), CH3CH(CH3)CH2. 

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