Bovine papillomavirus type

To date, the most successful antiviral targets have been directed against viral-specific enzymes. Therefore, the ATPase and helicase activities of the El protein are attractive targets. Papillomavirus DNA replication may also be inhibited by compounds that interfere with the ability of El to bind DNA or to interact with the E2 protein. This chapter will describe a method to assay for specific El DNA binding and cooperative origin binding with the E2 protein and a method to transiently assay papillomavirus DNA replication. The methods described are for bovine papillomavirus type 1 (BPV-1), which has been the molecular prototype of the papillomaviruses. However, the methods can easily be adapted to assay for human papillomavirus replication. 

Li, R. and Botchan, M. R. (1994) Acidic transcription factors alleviate nucleo-some-mediated repression of DNA replication of bovine papillomavirus type 1. Proc. Natl. Acad. Sci. USA 91,7051-7055. 

Natural high-molecular weight sulphated or sulphonated PS, such as cellulose sulphate and dextran sulphate, may be useful non-toxic microbicidal compounds that are active against a variety of sexually-transmitted disease agents, including bovine papillomavirus type 1 and human papilloma virus type 11 and type 40 [111],... 

Another type of biosensor widely used is the DNA hybridization sensor. In this type of sensor, a DNA probe is immobilized on the transducer of the sensor. When the complementary strand hybridizes to the probe, a signal is generated that is then detected. As an example, the sensor developed by Nascimento et al. [19] detected bovine papillomavirus DNA electiochemically on a sensor functionalized with a specific probe of 27 bases. 

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