Bovine corneal opacity and permeability

Gautheron, P.D., Dukic, M., Alix, D. and Sina, J.F. (1992). Bovine corneal opacity and permeability test An in vitro assay of ocular irritation. Fund. Appl. Toxicol. - Cut. Ocular Toxicol. 2 107-117. [Pg.681]

The Bovine Corneal Opacity and Permeability Test Method... [Pg.173]

The Bovine Corneal Opacity and Permeability test method (BCOP) is based on normal physiological and biochemical function of the bovine cornea in vitro. For the BCOP, the eyes are taken from animals slaughtered for human consumption. In this test method (OECD Test Guideline, TG 437), the damage caused by the test substance is assessed by quantitative measurements of changes in corneal opacity and permeability with an opacitometer or a visible... [Pg.173]

Top-down approach1 Identification of serious eye damage Positive results lead to serious eye damage (GHS Cat. 1) classification Bovine Corneal Opacity and permeability (BCOP) test Validated and adopted (OECD TG 437) Applicable to substances and formulations. Positive results obtained with alcohols or ketones should be interpreted cautiously due to risk of overprediction. [Pg.174]

Bottom-up approach13 Identification of non-classified test materials Bovine Corneal Opacity and permeability test Validated and accepted0 (OECD TG 437) Applicable to substances and formulations. [Pg.174]

Cooper KJ, Earl LK, Harbell J, Raabe H (2001) Prediction of ocular irritancy of prototype shampoo formulations by the isolated rabbit eye (IRE) test and bovine corneal opacity and permeability (BCOP) assay. Toxicology In Vitro 15 95-103... [Pg.196]

Kolle SN, Kandarova H, Wareing B, Ravenzwaay B, Landsiedel R (2011) In-house validation of the EpiOcular Eye Irritation Test and its combination with the bovine corneal opacity and permeability test for the assessment of ocular irritation. ATLA 39 365-387... [Pg.197]

None of the in vitro alternative eye tests has proven applicable as a valid replacement for the Draize eye irritation test or has been acceptable for regulatory purposes (Table 5), though some are considered either reliable or reproducible. The most frequently used test has been the ex vivo bovine cornea opacity and permeability assay. The newer human corneal equivalents system, an in vitro culture of immortalized human corneal cells that develops into... [Pg.2727]

Additional in vitro models and assays for eye irritation testing have been proposed in the past. An example is the porcine-based corneal opacity and permeability (PCOP) assay to predict eye irritation of water-soluble cosmetic ingredients [99]. Porcine corneas were chosen due to their advantages in comparison to the bovine corneas such as no concerns about encephalopathy diseases, regular supply of eyes with an acceptable level of quality from a slaughterhouse, more closely related to the human cornea, and their use in ophthalmic research. Using a modified holder and adapted experimental procedures 50 cosmetic ingredients were tested and compared to the in vivo Draize MAS scores. Based on their results, the study concluded that the PCOP could better predict the irritation classes than the BCOP assay. [Pg.190]

MDCK cells/dye diffusion Cell and tissue physiology Epidermal slice/electrical conductivity Rabbit ileum/contraction inhibition Bovine cornea/corneal opacity Proptoses mouse eye/permeability test Inflammation/immunity Chorioallantoic membrane (CAM)... [Pg.1131]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...