Bovine aortic endothelial cells BAECs

Abstract Cyclosporine A (CsA) therapy is associated with side effects related to oxidative stress. We characterized the reactive oxygen and nitrogen species produced in the extracellular and intracellular compartments of bovine aortic endothelial cells (BAEC) exposed to CsA. CsA induced a dose-dependent increase of the intracellular oxidation of the NO-sensitive fluorescent probe DAF-2/DA. In agreement with this, CsA produced a dose-dependent accumulation of nitrites in the supernatants of BAEC. In contrast, in BAEC treated with CsA, the presence of superoxide anion could only be detected in the intracellular compartment, as measured by the oxidation of dihydroethidium. The formation of peroxynitrite was assessed in the intracellular compartment, by flow cytometry with... 

Garcia-Cardena reported in 1996 that the tyrosine phosphorylation of eNOS in bovine aortic endothelial cells (BAEC) resulted in a decrease in enzyme activity whilst immunoprecipitation of caveolin-1, the predominant isoform in endothelial cells, resulted in co-immunoprecipitation of tyrosine phosphorylated eNOS [17]. Michel et al. (1997) further reported that eNOS, also in BAEC, was co-immunoprecipitaed by caveolin-1 antibodies whilst in rat myocytes eNOS was associated with caveolin-3, the cardiac muscle specific type, confirming that eNOS interaction with caveolin is not tissue specific [18]. Immunofluoresence studies in our group have shown that eNOS associates with caveolin-1 in human umbilical vein endothelial cells (HUVEC), and this interaction is abolished by bradykinin (Wyatt, Pedley Mann, 2001 unpublished data) (Figure 3). 

Hydrazinocurcuminoids modified from curcuminoids by Shim et al. [64] showed greater anti-proliferative potency than curcuminoids in bovine aortic endothelial cells (BAECs) (Table 7). In contrast to curcumin, which inhibited the proliferation of several epithelial and fibroblast cells non-selectively, hydrazinocurcuminoids showed specificity against endothelial cells. Thus, proper modification on the linker between the two phenyl rings cannot only enhance the anti-angiogenic activity but also the specificity. The rank order of the potencies of the curcuminoids was 1 > 2 > 3. The hydrazinocurcuminoids showed the same trend. Therefore, the elimination of methoxy groups from the phenyl rings resulted in decreased anti-angiogenic potency. 

Chen, C. Y, Barron, J. A., and Ringeisen, B. R. 2006. Cell patterning without chemical surface modification Cell-cell interactions between printed bovine aortic endothelial cells (BAEC) on a homogeneous cell-adherent hydrogel. Appl SurfSci, 252,8641-5. 

MDCK (Madin-Darby canine kidney) cells are derived from distal tubules, whereas LLC-PKi are from proximal tubes. b BMEC (brain microvessel endothelial cells) are isolated from capillaries. BPAEC (bovine pulmonary artery endothelial cells), BAEC (bovine aortic endothelial cells), and HUVEC (human umbilical vein endothelial cells) are large vessel endothelia. 

Fig. 31 Rate of surface-mediated protein C activation for TM reconstituted into a poly (acrylatePC) HBM at a protein surface coverage of 170fmolcm 2 (sample) compared to confluent endothelial cell monolayers (HDMEC, human dermal microvascular endothelial cells HUVEC, human umbilical vein endothelial cells BAEC, bovine aortic endothelial cells). Reprinted with permission from [139], Copyright 2002, American Chemical Society...

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